Figure 4: In vivo antibody expression analysis of 1G7C2_hG1-LALA in BALB/c mice. (A) A total of 100 μg of pFUSE-1G7C2-hVH-LALA and pFUSE-1G7C2-hVL expressing plasmids were injected into each BALB/c mice (n=8). Injection of pFUSE vector was used as negative control (n=3). Serums were collected at each time point and measured by human IgG ELISA. ***P<0.001. (B) The pooled mouse serum on day 7 was investigated by immunofluorescence assay of four DENV serotypes. (C) Pooled mouse serum of each time point was subjected to FRNT against DENV-2. (D) The percent reduction by using the pooled mouse serum on day 7 against DENV-1, -3, and -4.