Figure 1: In vitro expression analysis of the secreted HuMAb against DENV. (A) Immunofluorescence assay demonstrated that the secreted 1G7C2_hG1 and 1G7C2_hG1-LALA reacted to the envelope protein of four DENV serotypes. pFUSE_hG1 was used as negative control. (B) Human IgG in culture supernatant was quantified by ELISA. The values are presented as mean ± SD. (C) Western blot analysis of human IgG heavy- and light-chain peptides from culture supernatant 1G7C2_hG1 and 1G7C2_hG1-LALA under reducing condition. The original photograph is shown in [Supplementary Figure 1C].