Figure 5: Parasite load in rDIM-1bm-immunized Mastomys coucha subsequently challenged with third-stage larvae of filarial parasite Brugia malayi (L3) infection. (A, C & E) Status of microfilaremia in 10 μL tail blood of Mastomys coucha immunized with rDIM-1bm for 3-dose, 3-week and 6-week shedule and subsequently inoculated with L3 of Brugia malayi. (B, D & F) Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. Parasites were recovered from all the animals and analyzed. Student’s t test was used. *P<0.05, **P<0.01, ***P<0.001 vs. Non- Im+L3. The experiment was repeated twice on each group of 5-6 animals.