Figure 4: Humoral immune responses of immunized animals. (A, E, I) IgG and its subclasses, (B, F, J) IgE, (C, G, K) IgM and (D, H, L) IgA in sera of Mastomys coucha immunized with rDIM-1bm for 3 doses, 3 and 6 weeks and subsequently challenged with third-stage larvae of filarial parasite Brugia malayi (L3) infection. Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. Tukey’s multiple comparison test was used. *P<0.05, **P<0.01, ***P<0.001. Concentration of released cytokines are calculated by using values of standard cytokines with absorbance of released cytokines during experiment. The experiment was repeated twice on each group of 5-6 animals. Immunoglobulins G, A, M, E and IgG isotypes were measured using indirect ELISA; the concentrations were expressed as OD readings at 492 nm.