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  Indian J Med Microbiol
 

Figure 3: Effect of immunization and third-stage larvae of filarial parasite Brugia malayi (L3) infection on cytokine production. Thl (IFN-γ: A, G, M; TNF-α: B, H, N) and Th 2 (IL-4: C, I, O; IL-6: D, J, P; IL-10: E, K, Q; IL-13: F, L, R) cytokine release by splenocytes of Mastomys coucha immunized with rDIM-1bm for 3-dose, 3- and 6-week shedule and then challenged with L3 infection. Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. The cells of animals were stimulated with LPS (1 μg/mL) and rDIM-1bm (1 μg/mL) in vitro; concentration of released cytokines were calculated by using values of standard cytokines with absorbance of released cytokines during experiment (expressed as pg protein/mL); Tukey’s multiples comparison test was used, *P<0.05, **P<0.01, ***P<0.001; The experiment was repeated twice on each group of 5-6 animals. Unst: Unstimulated, LPS: Lipopolysaccharide, p.f.i: post first immunization.

Figure 3: Effect of immunization and third-stage larvae of filarial parasite <i>Brugia malayi</i> (L3) infection on cytokine production. Thl (IFN-γ: A, G, M; TNF-α: B, H, N) and Th 2 (IL-4: C, I, O; IL-6: D, J, P; IL-10: E, K, Q; IL-13: F, L, R) cytokine release by splenocytes of <i>Mastomys coucha</i> immunized with rDIM-1bm for 3-dose, 3- and 6-week shedule and then challenged with L3 infection. Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. The cells of animals were stimulated with LPS (1 μg/mL) and rDIM-1bm (1 μg/mL) <i>in vitro;</i> concentration of released cytokines were calculated by using values of standard cytokines with absorbance of released cytokines during experiment (expressed as pg protein/mL); Tukey’s multiples comparison test was used, *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001; The experiment was repeated twice on each group of 5-6 animals. Unst: Unstimulated, LPS: Lipopolysaccharide, p.f.i: post first immunization.