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  Indian J Med Microbiol
 

Figure 2: Cellular immune responses of rDIM-1bm-immunized animals challenged with third-stage larvae of filarial parasite Brugia malayi (L3) infection. (A-C) proliferative response of splenocytes by LTT; (D-F) phagocytic activity of peritoneal macrophages by MAT and (G-I) NO production by peritoneal macrophages of Mastomys coucha immunized with rDIM-1bm for 3-dose, 3- and 6-week shedules and subsequently challenged with L3. Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. The cells of animals were stimulated with LPS (1 μg/mL) and rDIM-1bm (1 μg/mL) in vitro. Tukey’s multiples comparison test was used. *P<0.05, **P<0.01, ***P<0.001. The experiment was repeated twice on each group of 5-6 animals. ConA: Concanavalin A, Unst: Unstimulated, LPS: Lipopolysaccharide, p.f.i: Post first immunization; CMP: Count per minute; LTT: Lymphocyte transformation test; MAT: Macrophage activation test; NO: Nitric oxide.

Figure 2: Cellular immune responses of rDIM-1bm-immunized animals challenged with third-stage larvae of filarial parasite <i>Brugia malayi</i> (L3) infection. (A-C) proliferative response of splenocytes by LTT; (D-F) phagocytic activity of peritoneal macrophages by MAT and (G-I) NO production by peritoneal macrophages of <i>Mastomys coucha</i> immunized with rDIM-1bm for 3-dose, 3- and 6-week shedules and subsequently challenged with L3. Non-Im: Non-immunized; rDIM-1bm-Im: rDIM-1bm immunized; Non-Im+L3: Non-immunized+L3 infected; rDIM-1bm-Im: rDIM-1bm immunized+L3 infected. The cells of animals were stimulated with LPS (1 μg/mL) and rDIM-1bm (1 μg/mL) <i>in vitro</i>. Tukey’s multiples comparison test was used. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. The experiment was repeated twice on each group of 5-6 animals. ConA: Concanavalin A, Unst: Unstimulated, LPS: Lipopolysaccharide, p.f.i: Post first immunization; CMP: Count per minute; LTT: Lymphocyte transformation test; MAT: Macrophage activation test; NO: Nitric oxide.