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Figure 5: FMLP50 inhibited expression of PPAR-γ's target protein in differentiated 3T3-L1 cells. 3T3-L1 cells were maintained in culture medium (control)and induced adipogenesis with differentiation medium (DM). After treatment with DMSO or FMLP50 for 8 d, total proteins were extracted using RIPA buffer,and analysis by western blot. The results showed that FMLP50 inhibited expression of PPAR-γ's target protein at the time point of 8 d. Values present as mean±SD (n=3), *as P<0.05, **as P<0.01. |
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