Figure 4: FMLP50 inhibited expression of PPAR-γ at 24 h (A) and 8 d (B). 3T3-L1 cells were maintained in culture medium (control) and induced adipogenesis with differentiation medium (DM). The cells were treated with DMSO or FMLP50 for 24 h and 8 d, total proteins and RNA were extracted using RIPA buffer and Trizol reagent, and analysis by western blot and quantitative real-time PCR. The results show that fermented extract inhibited expression of PPAR-γ both at time point of 24 h (A, C) and 8 d (B, D). Values present as mean±SD (n=3), *as P<0.05, **as P<0.01.