Figure 5: SIRT1 and ADAM10 protein levels of cells pretreated with EtOAc extract of H. formicarum Jack. prior exposure to 400 μM H₂O₂. SIRT1 and ADAM10 protein expressions in 400 μM H₂O₂-induced cells were recovered by the pretreatment of EtOAc plant extract determined by Western blot analysis. Densitometric analysis of protein bands were presented in the graph as the ratio to β-actin. The results are expressed as mean±S.E.M. of three independent experiments, *P<0.05 and **P<0.01 vs. control and ^## P<0.01 vs. 400 μM H₂O₂-treated group.