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  Indian J Med Microbiol
 

Figure 4: Mitochondria related proteins levels in high glucose-induced HK-2 cells. (A, B) High glucose up-regulated the mRNA expressions of Drp1 and PGC-1 α. Fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose induced Drp1 and PGC-1 α mRNA transcriptions. *: P<0.05 vs. 5 mM glucose group; #: P< 0.05 vs. 30 mM glucose group; (C) High glucose increased the expression of Drp1 protein, and fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose-induced Drp1 protein expression. **: P <0.01 vs. 5 mM glucose group; ##: P= 0.001 vs. 30 mM glucose group; (D) High glucose up-regulated the expression of PGC-1α protein, and fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose-induced PGC-1 α expression. @: P = 0.01 vs. 5 mM glucose group; $: P <0.01 vs. 30 mM glucose group.

Figure 4: Mitochondria related proteins levels in high glucose-induced HK-2 cells.
(A, B) High glucose up-regulated the mRNA expressions of <i>Drp1</i> and <i>PGC-1</i> α. Fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose induced <i>Drp1</i> and <i>PGC-1</i> α mRNA transcriptions. *: <i>P</i><0.05 <i>vs</i>. 5 mM glucose group; #: <i>P</i>< 0.05 <i>vs</i>. 30 mM glucose group; (C) High glucose increased the expression of Drp1 protein, and fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose-induced Drp1 protein expression. <sup>**</sup>: <i>P</i> <0.01 <i>vs</i>. 5 mM glucose group; <sup>##</sup>: <i>P</i>= 0.001 <i>vs</i>. 30 mM glucose group; (D) High glucose up-regulated the expression of PGC-1α protein, and fasudil and ROCK1 siRNA pretreatment significantly antagonized high glucose-induced <i>PGC-1</i> α expression. @: <i>P</i> = 0.01 <i>vs</i>. 5 mM glucose group; $: <i>P</i> <0.01 <i>vs</i>. 30 mM glucose group.