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  Indian J Med Microbiol
 

Figure 2: Relative mRNA level of MnSOD2. HK-2 cells were pre-treated with 10 μmol/ L fasudil for 30 min and then cultured with 30 mM glucose for 48 h. HK-2 cells were transfected with ROCK1 siRNA and sc-siRNA, and then cultured with 30 mM glucose. Realtime PCR analysis showed that high glucose treatment up-regulated the transcriptions of MnSOD2, while treatment with fasudil and ROCK1 siRNA significantly attenuated high glucose-induced MnSOD2 mRNA expression. *:P<0.001 vs. 5 mM glucose group; #: P<0.05 vs. 30 mM glucose group; &: P<0.001 vs. 30 mM glucose group.

Figure 2: Relative mRNA level of <i>MnSOD2</i>.
HK-2 cells were pre-treated with 10 μmol/ L fasudil for 30 min and then cultured with 30 mM glucose for 48 h. HK-2 cells were transfected with ROCK1 siRNA and sc-siRNA, and then cultured with 30 mM glucose. Realtime PCR analysis showed that high glucose treatment up-regulated the transcriptions of <i>MnSOD2</i>, while treatment with fasudil and ROCK1 siRNA significantly attenuated high glucose-induced <i>MnSOD2</i> mRNA expression. *:<i>P</i><0.001 <i>vs</i>. 5 mM glucose group; #: <i>P</i><0.05 <i>vs</i>. 30 mM glucose group; &: <i>P</i><0.001 <i>vs</i>. 30 mM glucose group.