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Figure 1: Upregulation of MYPT1 protein in HK-2 cells induced by high glucose. The Rho kinase signal pathway was involved in high glucose-induced mitochondrial-mediated oxidative damage and apoptosis in HK-2 cells. HK-2 cells were incubated with 5 mM glucose, 30 mM glucose and 30 mM mannitol for 48 h (n=3). Western blots showed that treatment with 30 mM glucose significantly increase the expression of MYPT1 protein. *: P<0.01 vs. 5 mM glucose control group; #: P = 0.01 vs. 30 mM glucose group. |
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