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  Indian J Med Microbiol
 

Figure 2: Temperature profiles of the derivative melt curves of the 12S rRNA gene for B. malayi (Bm), B. pahangi (Bp), and D. immitis (Di). The amplicons of B. malayi from 10 μL DBS, soaked in 200 μL TE buffer for 1 h and 20 μL DBS, soaked in 200 μL TE buffer, overnight revealed a similar variation in melting peak at 74.84 °C (10 μL DBS, 1 h and 20 μL DBS, overnight) and 20 μL DBS, soaked in 200 μL TE buffer, 1 h showed a peak at 75.81 °C (20 μL DBS, 1 h). However, B. malayi from 10 μL DBS, soaked in200 μL TE buffer, overnight showed no melting peak.

Figure 2: Temperature profiles of the derivative melt curves of the <i>12S rRNA</i> gene for <i>B. malayi</i> (Bm), <i>B. pahangi</i> (Bp), and <i>D. immitis</i> (Di).
The amplicons of <i>B. malayi</i> from 10 μL DBS, soaked in 200 μL TE buffer for 1 h and 20 μL DBS, soaked in 200 μL TE buffer, overnight revealed a similar variation in melting peak at 74.84 °C (10 μL DBS, 1 h and 20 μL DBS, overnight) and 20 μL DBS, soaked in 200 μL TE buffer, 1 h showed a peak at 75.81 °C (20 μL DBS, 1 h). However, B. malayi from 10 μL DBS, soaked in200 μL TE buffer, overnight showed no melting peak.