Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 13  |  Issue : 12  |  Page : 557--565

Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling


Lu Guo1, Jum Soon Kang1, Young Hoon Park1, Beong Il Je1, Dae Youn Hwang2, Woo Hong Joo3, Young Whan Choi4 
1 Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Republic of Korea
2 Department of Biomaterials Science, Pusan National University, Miryang 50463, Republic of Korea
3 Department of Biology and Chemistry, Changwon National University, Changwon 51140, Republic of Korea
4 Department of Horticultural Bioscience; Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea

Correspondence Address:
Young Whan Choi
Department of Horticultural Bioscience; Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463
Republic of Korea

Objective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais. Methods: Dried mulberry leaves, dried mulberry leaves with 50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25 °C, after which the dried mulberry leaves and fermented product were extracted with 70% ethanol and subjected to high performance liquid chromatography (HPLC). The contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid were determined. We then used the 3T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro. Results: HPLC showed that fermentation changed the contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid. Furthermore, fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves, fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption. Additionally, fermented dried mulberry leaves with 50% raw silkworm pupa inhibited PPAR-γ signaling. Conclusions: Fermentation with Cordyceps militaris enhanced anti-adipogenesis efficacy of mulberry leaves.


How to cite this article:
Guo L, Kang JS, Park YH, Je BI, Hwang DY, Joo WH, Choi YW. Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling.Asian Pac J Trop Med 2020;13:557-565


How to cite this URL:
Guo L, Kang JS, Park YH, Je BI, Hwang DY, Joo WH, Choi YW. Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling. Asian Pac J Trop Med [serial online] 2020 [cited 2020 Nov 27 ];13:557-565
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=12;spage=557;epage=565;aulast=Guo;type=0