Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 3  |  Page : 123--129

Isolation and structural elucidation of antifungal compounds from Curcuma amada


Jesmin Akter1, Kensaku Takara2, Md. Zahorul Islam2, Md. Amzad Hossain3, Ayako Sano2, De-Xing Hou1 
1 The United Graduate School of Agricultural Sciences, Kagoshima University, Japan
2 Faculty of Agriculture, University of the Ryukyus, Japan
3 The United Graduate School of Agricultural Sciences, Kagoshima University; Faculty of Agriculture, University of the Ryukyus, Japan

Correspondence Address:
Md. Amzad Hossain
Professor, Subtropical Field Science Center, Faculty of Agriculture, University of the Ryukyus, Senbaru-1, Nishihara City, Okinawa 903-0213
Japan

Objective: To isolate and identify the antifungal compounds from Curcuma amada. Methods: The antifungal activity was measured by the diameter of colonies grown on Petri dish, microscopic observation, and CLSI microdilution methods. The antifungal compounds were isolated through bioactivity guided purification by using silica gel and high-performance liquid chromatography. Structural identification of the antifungal compounds was conducted using 1H NMR, 13C NMR, and liquid chromatography-tandem mass spectrometry. Results: The purified antifungal compounds were zederone and furanodienone. These two compounds showed dose-dependent antifungal activity against Fusarium solani sensu lato. The concentration required for 50% growth inhibition (IC50) of FSSL ranged from 115 to 129 μM and 82 to 91 μM for zederone and furanodienone, respectively. Conclusion: This study suggested that the isolated compounds from Curcuma amada could be promising natural antifungal agents to control the diseases caused by Fusarium solani sensu lato.


How to cite this article:
Akter J, Takara K, Islam MZ, Hossain MA, Sano A, Hou DX. Isolation and structural elucidation of antifungal compounds from Curcuma amada.Asian Pac J Trop Med 2019;12:123-129


How to cite this URL:
Akter J, Takara K, Islam MZ, Hossain MA, Sano A, Hou DX. Isolation and structural elucidation of antifungal compounds from Curcuma amada. Asian Pac J Trop Med [serial online] 2019 [cited 2020 Oct 27 ];12:123-129
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=3;spage=123;epage=129;aulast=Akter;type=0