Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 3  |  Page : 106--116

Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract: In vitro and in vivo study


Nahla Deghbar1, Dalila Mezioug1, Touri Kahina1, Yacine-Miloud Medjdoub2, Chafia Touil- Boukoffa1 
1 Cytokines and NO Synthases-Immunity and Pathogeny team, Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria
2 Department of Thoracic Surgery, Mustapha Pacha Hospital, Algiers, Algeria

Correspondence Address:
Dalila Mezioug
Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, PB 32, El-Alia, 16111, Algiers
Algeria

Objective: To evaluate the in vitro and in vivo effect of the Algerian propolis ethanolic extract (EEP) against Echinococcus granulosus (E. granulosus) infection. Methods: In vitro scolicidal activity of EEP was investigated on the protoscolices of hydatid cyst. This in vitro study was conducted by using an in vivo assay. BALB/c mice were inoculated with E. granulosus and treated with propolis for three months. Hydatid cysts development was assessed. Nitric oxide (NO), tumor necrosis factor alpha (TNF-α) production and inducible NO synthase, NF-κB, and TNF-α spleen expression were estimated by Griess method and immunofluorescence respectively. Results: Our study revealed that EEP has a high scolicidal activity against E. granulosus. Oral administration of EEP decreased TNF-α, NF-κB and inducible NO synthase expression in the spleen tissues in the CE+EEP group, in comparison with the CE group. Concomitantly, EEP treatment caused an important systemic decrease in NO and TNF-α levels. These findings are associated with the reduction of CE development. Conclusions: This is the first report demonstrating with interest the antihydatic and immunomodulatory effects of the Algerian EEP, suggesting its therapeutic potential for the hydatid disease treatment.


How to cite this article:
Deghbar N, Mezioug D, Kahina T, Medjdoub YM, Touil- Boukoffa C. Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract: In vitro and in vivo study.Asian Pac J Trop Med 2019;12:106-116


How to cite this URL:
Deghbar N, Mezioug D, Kahina T, Medjdoub YM, Touil- Boukoffa C. Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract: In vitro and in vivo study. Asian Pac J Trop Med [serial online] 2019 [cited 2020 Oct 29 ];12:106-116
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=3;spage=106;epage=116;aulast=Deghbar;type=0