Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 1  |  Page : 32--37

Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco


Messaoudi Mouhcine1, Laglaoui Amin2, Amzazi Saaid4, Hammani Khalil3, Benbacer Laila4, El Mzibri Mohammed4 
1 Unité de Biologie et Recherche Médicale, CNESTEN; Laboratoire de Biochimie-Immunologie, Faculté des Sciences, Mohammed V University, Rabat, Morocco
2 Équipe de Recherche en Biotechnologies & Génie des Biomolécules, Faculté des Sciences et Techniques de Tanger, Abdelmalek Essaadi University, Morocco
3 Laboratoire de Ressources Naturelles et Environnement, Faculté Polydisciplinaire de Taza, Sidi Mohammed Ben Abdellah University, Morocco
4 Unité de Biologie et Recherche Médicale, CNESTEN, Rabat, Morocco

Correspondence Address:
Dr. El Mzibri Mohammed
Biology and Medical Research Unit, CNESTEN, BP 1382 RP. 10001 Rabat
Morocco

Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander (N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bioassay on two human cancer cell lines, namely human colon adenocarcinoma cell line HT-29 and human breast cancer cell line MDA-MB-231. The antioxidant property of N. oleander extracts was assessed by DPPH scavenging and β -carotene bleaching tests. The agar disc diffusion method was used for the determination of antimicrobial activity against different strains. Results: Using cell viability indices, the WST-1 test revealed that both extracts reduced cell viability in both cell lines. Our results also showed that aqueous extract was more active than ethanolic extract, with IC50 values of (1.67±0.22) μg/mL and (2.36±0.44) μg/mL on MDA-MB-231 cells, and (2.89±0.35) μg/mL and (5.09±0.52) μg/mL on HT29 cells, respectively. The study of the antioxidant activity showed that N. oleander extracts had a considerable scavenging capacity and exerted a significant preventive effect against the oxidation of β -carotene by the peroxide radicals. However, the antibacterial test showed that both ethanolic and aqueous extracts of N. oleander had a moderate antibacterial effect limited only to Gram-positive bacteria. Conclusions: Our results shows that N. oleander aqueous and ethanolic extracts have significant cytotoxic activities against tumor cell lines and possesses a strong antioxidant capacity, suggesting the presence of active compounds in N. oleander leaves that could be a potential source of phytochemicals with high medicinal value to be used in cancer treatment and prevention.


How to cite this article:
Mouhcine M, Amin L, Saaid A, Khalil H, Laila B, Mohammed E. Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco.Asian Pac J Trop Med 2019;12:32-37


How to cite this URL:
Mouhcine M, Amin L, Saaid A, Khalil H, Laila B, Mohammed E. Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco. Asian Pac J Trop Med [serial online] 2019 [cited 2020 Oct 27 ];12:32-37
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=1;spage=32;epage=37;aulast=Mouhcine;type=0