Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 1  |  Page : 14--18

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus


Ryong Gong, Dae Young Lee, Jae Won Lee, Doo Jin Choi, Geum-Soog Kim, Sang Hyuk Lee, Young-Seob Lee 
 Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709, Korea

Correspondence Address:
Young-Seob Lee
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709
Korea

Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/ blaR1 gene expressions.


How to cite this article:
Gong R, Lee DY, Lee JW, Choi DJ, Kim GS, Lee SH, Lee YS. Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus.Asian Pac J Trop Med 2019;12:14-18


How to cite this URL:
Gong R, Lee DY, Lee JW, Choi DJ, Kim GS, Lee SH, Lee YS. Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus. Asian Pac J Trop Med [serial online] 2019 [cited 2020 Oct 25 ];12:14-18
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=1;spage=14;epage=18;aulast=Gong;type=0