Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 9  |  Page : 540--546

Nano chloroquine delivery against Plasmodium berghei NK65 induced programmed cell death in spleen


Satyajit Tripathy1, Sourav Chattopadhyay2, Sandeep Kr Dash3, Motlalepula G Matsabisa5, Somenath Roy5 
1 Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721102, West Bengal, India; Department of Pharmacology, University of the Free State, Bloemfontein 9300, Republic of South Africa
2 B.S. B. E. Indian Institute of Technology, Kanpur, Uttar Pradesh, India
3 Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721102; University of Gour Banga, Malda, West Bengal, India

Correspondence Address:
Dr. Motlalepula G Matsabisa
Department of Pharmacology, University of the Free State, PO Box 339, Bloemfontein 9300
Republic of South Africa
Dr. Somenath Roy
Professor, Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721 102, West Bengal
India

Objective: To compare the protective effects of chitosan-trypolyphosphate (CS-TPP) nanoparticle conjugated chloroquine(CQ) with effect of CQ alone on the reversal of splenic damages and induction of apoptosis. Methods: Different researches have been carried out to explore the potential role of chitosan based drug delivery system against parasitic diseases. After successive Plasmodium berghei NK65 parasiste infection by intraperitoneal injection in Swiss mice and subsequent parasite development, the ROS generation, anti-apoptotic and pro apoptotic protein levels in spleen were measured. To analyze caspases, flow cytometry study was performed with annexin V -FITC and with PI staining. Results: The results revealed that ROS mediated caspase 3 and 9 activation and the induction of apoptosis occurred during the parasitic infection. However, CS-TPP conjugated CQ was relatively better in reversing the splenic damage compared with similar effects of CQ alone. Conclusions: This study indicates that Plasmodium berghei NK65 induces apoptosis in the spleen. The study further shows that CS-TPP nanoparticles conjugation with CQ have positive influence on the recovery of damaged host’s system towards maintenance of normal homeostasis, and this is shown to be selective to CS-TPP conjugated CQ treated animals only.


How to cite this article:
Tripathy S, Chattopadhyay S, Dash SK, Matsabisa MG, Roy S. Nano chloroquine delivery against Plasmodium berghei NK65 induced programmed cell death in spleen.Asian Pac J Trop Med 2018;11:540-546


How to cite this URL:
Tripathy S, Chattopadhyay S, Dash SK, Matsabisa MG, Roy S. Nano chloroquine delivery against Plasmodium berghei NK65 induced programmed cell death in spleen. Asian Pac J Trop Med [serial online] 2018 [cited 2021 Sep 17 ];11:540-546
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=9;spage=540;epage=546;aulast=Tripathy;type=0