Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 4  |  Page : 285--291

Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.)


Ahlem Ben Sassi1, Sana Amroussi1, Malek Besbes1, Mahjoub Aouni1, Fethia Skhiri2 
1 Laboratory of Transmissible Diseases and Biologically Active Substances, Department of Microbiology, Faculty of Pharmacy, University of Monastir, Tunisia
2 Laboratory of Genetics, Biodiversity and Valorisation of Bioresources, Higher Institute of Biotechnology, University of Monastir, Tunisia

Correspondence Address:
Ahlem Ben Sassi
Laboratory of Transmissible Diseases and Biologically Active Substances, Department of Microbiology, Faculty of Pharmacy, Avicenne street, Monastir
Tunisia

Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.


How to cite this article:
Sassi AB, Amroussi S, Besbes M, Aouni M, Skhiri F. Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.).Asian Pac J Trop Med 2018;11:285-291


How to cite this URL:
Sassi AB, Amroussi S, Besbes M, Aouni M, Skhiri F. Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.). Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 31 ];11:285-291
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=285;epage=291;aulast=Sassi;type=0