Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 3  |  Page : 245--250

MiR-503 promotes wound healing of diabetic foot ulcer by targeting FBN1


Ming-Li Wang1, Jing Chen2, Yue Zhou2, Yu-Jie Zhao2, De-Rong Sun2, Qiang Wu3, Chang- Long Bi2 
1 Department of Endocrinology, the Fourth Afliated Hospital of Harbin Medical University, Harbin, China
2 Comprehensive Second Department, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, China
3 Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, China

Correspondence Address:
Chang- Long Bi
Comprehensive Second Department, the Fourth Affiliated Hospital of Harbin Medical University, Harbin
China
Qiang Wu
Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou
China

Objective: To highlight the relationship between miR-503 and wound healing of diabetic foot ulcer (DFU). Methods: Microarray analysis was used to detect the dysregulated miRNAs between the DFU tissues and normal tissues. The expression of miR-503 in tissues and serum of patients with DFU was detected by qRT-PCR technique. Then, CCK-8 assay was applied to determine the cell proliferation. TUNEL assay was used for assessing the apoptosis of cells after treatment with miR-503. Possible correlation between miR-503 and fbillin1 (FBN1) was predicted according to data accessed on RNA22 website online, and was detected for confirmation by luciferase reporter assay. Results: Microarray analysis showed that miR- 503 was significantly decreased in the DFU tissues compared with normal tissues. While marked increase in the expression of miR-503 in tissues and serum of patients with DFU was confirmed by qRT-PCR technique. Then, CCK-8 assay indicated that transfection of miR- 503 mimic obviously accelerated the cell proliferation. However, TUNEL assays suggested that miR-503 mimic inhibited the apoptosis of cells to improve the survival of fibroblasts. Besides, miR-503 AMO played a role in fibroblasts of DFU tissues exactly countering to miR-503 mimic treatment. It was predicted that MiR-503 is a complementary to the FBN1 by RNA22. Besides, SiRNA-FBN1 promoted the proliferation, but brought down the apoptosis of fibroblasts. Conclusions: MiR-503 regulates the function of fibroblasts and wound healing of patients with DFU by targeting FBN1 directly which provids a novel and critical target for diagnosis and treatment of DFU.


How to cite this article:
Wang ML, Chen J, Zhou Y, Zhao YJ, Sun DR, Wu Q, Bi C. MiR-503 promotes wound healing of diabetic foot ulcer by targeting FBN1.Asian Pac J Trop Med 2018;11:245-250


How to cite this URL:
Wang ML, Chen J, Zhou Y, Zhao YJ, Sun DR, Wu Q, Bi C. MiR-503 promotes wound healing of diabetic foot ulcer by targeting FBN1. Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 25 ];11:245-250
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=3;spage=245;epage=250;aulast=Wang;type=0