Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 3  |  Page : 240--244

Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells


You-Wei Huang1, Jia-Hong Chen1, Zi-Xi Qin1, Jie-Kun Chen1, Ren-Dong Hu1, Zheng Wu2, Xi Lin1 
1 Department of Pharmacology, Medical College, Jinan University, Guangzhou, China
2 Key laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou; Department of Developmental and Regenerative Biology, Jinan University, Guangzhou, China

Correspondence Address:
Xi Lin
Department of Pharmacology, Medical College, Jinan University, No.601, West Huangpu Avenue, Tianhe District, Guangzhou, Guangdong
China
Zheng Wu
Key laboratory for Regenerative Medicine, Ministry of Education, Jinan University, No.601, West Huangpu Avenue, Tianhe District, Guangzhou, Guangdong
China

Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS. Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.


How to cite this article:
Huang YW, Chen JH, Qin ZX, Chen JK, Hu RD, Wu Z, Lin X. Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells.Asian Pac J Trop Med 2018;11:240-244


How to cite this URL:
Huang YW, Chen JH, Qin ZX, Chen JK, Hu RD, Wu Z, Lin X. Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells. Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 29 ];11:240-244
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=3;spage=240;epage=244;aulast=Huang;type=0