Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 3  |  Page : 235--239

Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells


Na Cheng1, Xiao-Ran Liu2, Da-Wei Liu3, Fang Liu4, Jin Xiang5, Di Yang6, Guo-Qiang Zhao7 
1 Department of Pathology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
2 Emergency Department, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, China
3 Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
4 Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
5 Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong Province, China
6 Department of Pathology, Nanhai District People’s Hospital, Foshan 528200, Guangdong Province, China
7 Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province; Pathology Teaching and Research Section, Sun Yat-sen University Zhongshan School of Medicine, Guangzhou 510080, Guangdong Province, China

Correspondence Address:
Guo-Qiang Zhao
Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan Road, Guangzhou 510080, Guangdong Province, China; Pathology Teaching and Research Section, Sun Yat-sen University Zhongshan School of Medicine; No. 58 Zhongshan Road, Guangzhou 510080, Guangdong Province
China

Objective: To study the effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells. Methods: Regenerated tissue extracts after liver injury were used to induce SK-HEP1 cells after enrichment, their effects on the proliferation, differentiation, migration and invasion of SK-HEP1 cells were observed through in vitro cell culture, MTT, flow cytometry and transwell assays. Results: In response to the action of regenerated tissue extracts after liver injury, SK-HEP1 cells were blocked in G0/G1 phase, their growth rate was distinctly reduced. The number of SK-HEP1- fj colonies decreased. The migration ability of SK-HEP1 cells showed a decreased trend on day 7 and day 11 after induction. SK-HEPl’s invasion ability clearly decreased on days 7 and 11 after induction, especially on day 7. Conclusions: To a certain extent, regenerated tissue extracts after liver injury can inhibit the proliferation, differentiation, migration and invasion of hepatoma cells, showing an important potential of being a differentiating agent for the treatment of liver cancer.


How to cite this article:
Cheng N, Liu XR, Liu DW, Liu F, Xiang J, Yang D, Zhao GQ. Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells.Asian Pac J Trop Med 2018;11:235-239


How to cite this URL:
Cheng N, Liu XR, Liu DW, Liu F, Xiang J, Yang D, Zhao GQ. Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells. Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 25 ];11:235-239
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=3;spage=235;epage=239;aulast=Cheng;type=0