Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 12  |  Page : 688--692

Can miRNA712_3p be a promising biomarker for early diagnosis of toxoplasmosis?


Nermine Mogahed Fawzy Hussein Mogahed1, Safaa Ibrahim Khedr1, Rasha Abdelmawla Ghazala2, Inas Mohamed Masoud3 
1 Medical Parasitology Department, Faculty of Medicine, Alexandria University, Egypt
2 Medical Biochemistry Department, Faculty of Medicine, Alexandria University, Egypt
3 Pharmaceutical Chemistry, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt

Correspondence Address:
Nermine Mogahed Fawzy Hussein Mogahed
Medical Parasitology Department, Faculty of Medicine, Alexandria University
Egypt

Objective: To assess the role of miRNA712_3p as a specific biomarker in early detection of toxoplasmosis in plasma of mice acutely infected with Toxoplasma gondii. Methods: Real-time PCR was used to measure the level of miRNA712_3p in plasma of infected mice. Immune-competent and immune-suppressed mice were examined, three and five days post-infection. Results: Results revealed significant up-regulation of plasma miRNA712_3p in both immune- competent and immune-compromised groups in comparison to the control non-infected group. Additionally, an increase in the level of miRNA712_3p was noticed correspondently in the parasite density detected in liver impression smears. Conclusions: miRNA712_3p can be used as a novel biomarker for the detection of Toxoplasma gondii infection in both immune-competent and immune-compromised host.


How to cite this article:
Mogahed NF, Khedr SI, Ghazala RA, Masoud IM. Can miRNA712_3p be a promising biomarker for early diagnosis of toxoplasmosis?.Asian Pac J Trop Med 2018;11:688-692


How to cite this URL:
Mogahed NF, Khedr SI, Ghazala RA, Masoud IM. Can miRNA712_3p be a promising biomarker for early diagnosis of toxoplasmosis?. Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 24 ];11:688-692
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=12;spage=688;epage=692;aulast=Mogahed;type=0