Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 12  |  Page : 659--665

Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells


Husna Syakirah Ab Rahman1, Wan Suriyani Wan-Ibrahim2, Norzila Ismail2, Tuan Nadrah Naim Tuan Ismail3, Siti Farhanah Mohd-Salleh2, Michael Pak-Kai Wong1, Mohd Ridzuan Abdul Samad1, Mohd Nizam Md Hashim1 
1 Department of Surgery, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
2 Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
3 School of Dental Sciences, Universiti Sains Malaysia,16150 Kubang Kerian, Kelantan, Malaysia

Correspondence Address:
Wan Suriyani Wan-Ibrahim
Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 6150 Kubang Kerian, Kelantan
Malaysia

Objective: To identify the phytochemical compounds from Annona muricata (A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry (GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC50 value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC50 value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages.


How to cite this article:
Rahman HA, Wan-Ibrahim WS, Ismail N, Ismail TN, Mohd-Salleh SF, Pak-Kai Wong M, Samad MA, Hashim MM. Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells.Asian Pac J Trop Med 2018;11:659-665


How to cite this URL:
Rahman HA, Wan-Ibrahim WS, Ismail N, Ismail TN, Mohd-Salleh SF, Pak-Kai Wong M, Samad MA, Hashim MM. Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells. Asian Pac J Trop Med [serial online] 2018 [cited 2020 Oct 22 ];11:659-665
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=12;spage=659;epage=665;aulast=Rahman;type=0