| ORIGINAL ARTICLE |
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| Year : 2023 | Volume
: 16
| Issue : 3 | Page : 119-128 |
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Tapered optical fiber DNA biosensor for detecting Leptospira DNA
Jia-Yong Lam1, Mohd Hanif Yaacob2, Hui-Yee Chee3
1 Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2 Department of Computer and Communication Systems Engineering, Faculty of Engineering; Wireless and Photonics Networks Research Centre (WiPNET), Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3 Department of Medical Microbiology, Faculty of Medicine and Health Sciences; Wireless and Photonics Networks Research Centre (WiPNET), Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Correspondence Address:
Hui-Yee Chee
Department of Medical Microbiology, Faculty of Medicine and Health Sciences; Wireless and Photonics Networks Research Centre (WiPNET), Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
Malaysia
 Source of Support: This research was funded by Universiti Putra Malaysia through the Geran Inisiatif Putra Siswazah (GP-IPS/2019/9678200), Conflict of Interest: None
DOI: 10.4103/1995-7645.372293
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Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.
Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 µm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.
Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/µL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.
Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.
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