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Table of Contents
LETTER TO EDITOR
Year : 2021  |  Volume : 14  |  Issue : 7  |  Page : 330-332

ACE2 downregulation promotes thrombosis and cardiac injury in COVID-19 patients


1 Department of Pharmacology and Toxicology, Centre for Laboratory Animal Technology and Research; School of Pharmacy, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai, Tamil Nadu 600119, India; Chinese Association for Laboratory Animal Sciences, Chaoyang District, Beijing 100021, China
2 Department of Microbiology, SRM Medical College Hospital and Research Centre, Tamil Nadu 603211, India
3 Department of Internal Medicine, Sundaram Health Centre, Tamil Nadu 632102, India

Date of Submission08-May-2021
Date of Decision15-Jun-2021
Date of Acceptance16-Jun-2021
Date of Web Publication12-Jul-2021

Correspondence Address:
Dhanasekaran Sivaraman
Department of Pharmacology and Toxicology, Centre for Laboratory Animal Technology and Research; School of Pharmacy, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai, Tamil Nadu 600119, India; Chinese Association for Laboratory Animal Sciences, Chaoyang District, Beijing 100021, China

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1995-7645.320524

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How to cite this article:
Sivaraman D, Leela KV, Venugopal V. ACE2 downregulation promotes thrombosis and cardiac injury in COVID-19 patients. Asian Pac J Trop Med 2021;14:330-2

How to cite this URL:
Sivaraman D, Leela KV, Venugopal V. ACE2 downregulation promotes thrombosis and cardiac injury in COVID-19 patients. Asian Pac J Trop Med [serial online] 2021 [cited 2021 Jul 24];14:330-2. Available from: https://www.apjtm.org/text.asp?2021/14/7/330/320524

The outbreak of novel coronavirus disease (COVID-19) caused by SARS-CoV-2 severely challenges the economic stability of countries around the globe. Immune responses induced by active infection and vaccination have resulted in severe complications like thrombosis and cardiac injury in a measurable number of COVID-19 cases. Hence, knowledge of dissemination of these events is of clinical importance.

Several bioactive enzymes are involved in the entry, replication and assembly of SARS-CoV-2 virus in host cells, of which angiotensin-converting enzyme-2 (ACE2) plays a crucial role in mediating the pathogenicity of SARS-CoV-2. ACE2 is expressed in multiple organs including the lungs, heart, digestive tract, liver, brain, eyes, pancreas and kidney, etc. Other than the lungs, the expression of ACE2 is found immensely on pericytes, cardiomyocytes, cardiac fibroblast, and epicardial adipocytes of the cardiac tissue. ACE2 mediates versatile pharmacological actions such as regulation of cardiac physiology, anti-oxidant, anti-fibrotic and anti-hypertrophic properties.

Recent studies hypothesise that SARS-CoV-2 infection reduces the expression of ACE2 at the membrane level[1]. In vitro studies demonstrated that ACE2 was downregulated in SARS-CoV-2 infected cell lines[2]. It is well known that cardiomyocytes have a higher expression of ACE2 and hence any alteration in the level of ACE2 may have a negative impact on cardiac function. In COVID-19 patients, the condition of hypoxemia greatly fluctuates the dynamics of blood flow to the cardiac tissue, which preambles the crisis of necrosis. Depleted ACE2 doubles the chances of inflammation (cytokine storm) and subsequent thrombotic risk in COVID-19 patients[3].

In vivo studies showed that the decrease in ACE2 level proportionately increases the expression of C-X3-C motif chemokine ligand 1 (CX3CL1). CX3CL1 is a larger fractalkine protein in the family of chemokines that exist in two forms: a membrane bound and soluble form[4]. In addition to ACE2 depletion, release of cytokines like TNF-α and IL-1β aggravates the expression of CX3CL1 on the endothelial matrix. Overexpression of CX3CL1 hastens the recruitment of T-cells and monocytes which contributes to the release of IL-1, IL-6, IL-10 and TNF-α in the microvascular environment. CX3CL1 exhibits direct involvement in the activation of platelets that upregulates the expression of P-selectin. Release of P-selectin through sequential degranulation of platelets triggers the episode of endotheliopathy and also promotes clot formation. Being a multifactorial component, CX3CL1 is now considered as a potential biomarker in ascertaining the severity of the cases. Available clinical data strongly correlates that patients with severe COVID-19 symptoms are observed with a two-fold increase in CX3CL1 level than in milder groups[5].

Limited expression of ACE2, in turn, destabilises the renin angiotensin aldosterone system mechanism, which leads to accumulation of angiotensin II (AII). Profound increase in AII primarily responsible for vasoconstriction and for the production of reactive oxidative species. Reactive oxygen species are unstable moieties that quench the lipoidal layer of the healthy cells and thereby promote organ failure. Release of aldosterone guided by overwhelming A II further upregulates the protein-C receptors which mediate hypercoagulable thrombotic events like DIC, pulmonary embolism and venous thrombosis in COVID-19 patients[6], as elucidated in [Figure 1].
Figure 1: Downregulation of ACE2 and its pathological implications in the event of thrombosis and cardiac injury in COVID-19.

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A drastic sweep in cytokine storms is one of the major limitation factors observed in the SARS-CoV-2 infection. Massive overproduction of certain inflammatory cytokines upsurge the probability of cardiovascular disease such as hypotension, left ventricular hypertrophy and tachycardia. Clinical recommendation of renin angiotensin aldosterone system inhibitors may halt the progression of thrombotic events, meanwhile, therapeutics that selectively block CX3CL1 type chemokines may widen the scope of alternate strategy in the treatment of COVID-19 patients. Angiotensin II receptor blocker (ARB) like losartan reveals a higher level of safety in patients with COVID-19 associated respiratory failure[7]. Results of three tire cohort clinical study shows that treatment with ARB’s exhibit significant clinical improvement in elderly COVID-19 patients[8].

Data from preclinical study emphasise that administration of angiotensin-converting enzyme inhibitor (ACEI-lisinopril) in rats upsurge the expression of cardiac ACE2 along with decreased plasma All level. Comparatively treatment with losartan (ARB) documents increased expression of cardiac ACE2 and its related activity in the experimental animals[9]. Outcome of meta-analysis over 1 664 (101 949 patients) published records acknowledge the protective benefits of using ACEIs and ARBs among COVID-19 patients with hypertension[10]. Despite clinical recommendations, large scale randomised trials are mandated to confirm the clinical implication of availing ACEIs and ARBs therapy in the treatment of COVID-19.

Conflict of interest statement

The authors declare no competing interests.

Acknowledgements

The authors thank the Indian Council of Medical Research (ICMR), Government of India, New Delhi, India.

Authors’ contributions

All authors contributed equally in preparation of this article.

 
  References Top

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Davidson AM, Wysocki J, Batlle D. Interaction of SARS-CoV-2 and other coronavirus with ACE (angiotensin-converting enzyme)-2 as their main receptor: Therapeutic implications. Hypertension 2020; 76(5): 1339-1349.  Back to cited text no. 1
    
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Glowacka I, Bertram S, Herzog P, Pfefferle S, Steffen I, Muench MO, et al. Differential downregulation of ACE2 by the spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus NL63. J Virol 2010; 84(2): 1198-1205.  Back to cited text no. 2
    
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Kumar A, Narayan RK, Kumari C, Faiq MA, Kulandhasamy M, Kant K, et al. SARS-CoV-2 cell entry receptor ACE2 mediated endothelial dysfunction leads to vascular thrombosis in COVID-19 patients. Med Hypothes 2020; 145: 110320. doi: 10.1016/j.mehy.2020.110320.  Back to cited text no. 6
    
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Ferrario CM, Jessup J, Chappell MC, Averill BD, Brosnihan BK, Tallant AE, et al. Effect of angiotensin-converting enzyme inhibition and angiotensin II receptor blockers on cardiac angiotensin-converting enzyme 2. Circulation 2005; 111(20): 2605-2610. doi: 10.1161/ CIRCULATIONAHA.104.510461.  Back to cited text no. 9
    
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Baral R, Tsampasian V, Debski M, Moran B, Garg P, Clark A, et al. Association between renin-angiotensin-aldosterone system inhibitors and clinical outcomes in patients with COVID-19: A systematic review and meta-analysis. JAMA Netw Open 2021; 4(3): e213594. doi:10.1001/ jamanetworkopen.2021.3594.  Back to cited text no. 10
    


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