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ORIGINAL ARTICLE
Year : 2020  |  Volume : 13  |  Issue : 3  |  Page : 131-136

Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico

Nallely Rivero-Perez1, Juan Ocampo-López1, Benjamín Valladares-Carranza2, Fabián R Gómez de Anda1, Francisco J Peña Jiménez3, Victor M Martínez Juárez1, Armando Peláez Acero1, José I Olave Leyva1, Deyanira Ojeda-Ramírez1, Adrian Zaragoza-Bastida1
1 Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuaria, Universidad Autónoma del Estado de Hidalgo, Av.Universidad Km 1, Ex-Hda. de Aquetzalpa, C.P. 43600 Tulancingo, Hidalgo, México
2 Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Panamericana Toluca-Atlacomulco, C.P. 50200 Toluca, Estado de México, México
3 Centro Universitario del Sur/División de Ciencias de la Salud/Departamento de Ciencias Clínicas/Hospital Veterinario CUSUR Universidad de Guadalajara, Av. Enrique Arreola Silva No. 883, colonia centro C.P. 49000, Ciudad Guzmán, Jalisco, México

Correspondence Address:
Adrian Zaragoza-Bastida
Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuaria, Universidad Autónoma del Estado de Hidalgo, Av.Universidad Km 1, Ex-Hda. de Aquetzalpa, C.P. 43600 Tulancingo, Hidalgo
México
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Source of Support: The authors would like to acknowledge the financial assistance from the Secretary of Research of Universidad Autónoma del Estado de Hidalgo (UAEH) through the project “Epidemiological surveillance of the genus Mycobacterium in the species Didelphis virginiana (Tlacuache) of the valley of Tulancingo Hidalgo” with number UAEH/DI/ICAp/MVZ/6, Conflict of Interest: None


DOI: 10.4103/1995-7645.278098

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Objective: To isolate and identify the exact species of the genus Mycobacterium from Didelphis (D.) virginiana, and the direct implications of this bacterium to public health and veterinary medicine. Methods: Thirty-one D. virginiana were captured and necropsied in Hidalgo, Mexico. Tissue samples were collected to culture mycobacteria present and examine individual specimens’ histopathology. Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques. Results: Three strains were isolated and identified as Mycobacterium (M.) avium subsp. hominissuis by utilizing M. avium complex- specific primers. Granulomatous lesions were observed in the subpleural zone (granuloma grade Π ) and bronchial (granuloma grade I ) of the lungs of D. virginiana with positive isolation. Conclusions: Three strains of M. avium subsp. hominissuis, from lung tissue samples of D. virginiana were identified. This subspecies of M. avium has important implications in public health and veterinary medicine.


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