|LETTER TO EDITOR
|Year : 2020 | Volume
| Issue : 2 | Page : 94-96
First evidence of Bartonella phoceensis and Candidatus Mycoplasma haemomuris subsp. ratti in synanthropic rodents in Malaysia
Van Lun Low1, Tiong Kai Tan2, Jamaiah Ibrahim3, Sazaly AbuBakar4, Yvonne Ai Lian Lim5
1 Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Malaysia
2 Department of Parasitology, Faculty of Medicine, University of Malaya, Malaysia
3 Faculty of Medicine and Defence Health, National Defence University of Malaysia, Malaysia
4 Tropical Infectious Diseases Research and Education Centre (TIDREC); Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia
5 Department of Parasitology, Faculty of Medicine; Centre of Excellence for Research in AIDS (CERIA), University of Malaya, Malaysia
|Date of Submission||12-Aug-2019|
|Date of Decision||14-Dec-2019|
|Date of Acceptance||16-Dec-2019|
|Date of Web Publication||14-Jan-2020|
Tiong Kai Tan
Department of Parasitology, Faculty of Medicine, University of Malaya
Van Lun Low
Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Malaysia
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Low VL, Tan TK, Ibrahim J, AbuBakar S, Lim YA. First evidence of Bartonella phoceensis and Candidatus Mycoplasma haemomuris subsp. ratti in synanthropic rodents in Malaysia. Asian Pac J Trop Med 2020;13:94-6
|How to cite this URL:|
Low VL, Tan TK, Ibrahim J, AbuBakar S, Lim YA. First evidence of Bartonella phoceensis and Candidatus Mycoplasma haemomuris subsp. ratti in synanthropic rodents in Malaysia. Asian Pac J Trop Med [serial online] 2020 [cited 2023 Jun 1];13:94-6. Available from: https://www.apjtm.org/text.asp?2020/13/2/94/275418
Rodent-borne leptospirosis is by far the most common bacterial zoonosis and it is an important emerging global public health concern in Southeast Asia. Bacterial pathogens associated with rodents, especially those that live in close association with humans have been underreported. To fill this knowledge gap, the present study was undertaken to explore other neglected disease agents that can naturally infect synanthropic rodents. Both Bartonella and Mycoplasma pose major health threats to various animal hosts and they are also emerging zoonoses and pathogens of public health concern. With these in mind, we aimed to detect the presence of Bartonella and Mycoplasma bacteria in synanthropic rodents from a densely populated capital city of Malaysia, Kuala Lumpur.
Rodent collection has been described elsewhere in detail. Briefly, a total of 134 synanthropic rodents comprising Rattus (R.) rattus diardii, R. norvegicus, R. argentiventer, R. tiomanicus, and R. exulans were trapped from two human populated areas (Sentul and Chow Kit) in Kuala Lumpur using steel wire traps. Blood was collected from the heart using a needle and syringe and placed into EDTA tube. Genomic DNA was extracted from whole blood using the QIAamp DNA Blood Mini Kit (QIAGEN Inc.,Valencia, CA).
Bartonella DNA detection was performed using polymerase chain reaction (PCR) with primers (325-5’CTT CAG ATG ATG ATC CCA AGC CTT TTG GCG-3’), and (1100-5’ GAA CCG ACG ACC CCC TGC TTG CAA AGC-3’) which amplify a portion of the 16S-23S rRNA intergenic spacer region. PCR amplification was performed in a final volume of 25 μL containing 25-50 ng genomic DNA, 12.5 μL of MyTaq Red Mix (Bioline Reagents Ltd., London, UK) and 10 pmol of each forward and reverse primer. PCR was performed using the Applied Biosystems Veriti 96-Well Thermal Cycler (Applied Biosystems, Inc., Foster City, CA) with the following thermal protocol: initial denaturation at 95 °C for 2 min followed by 55 cycles of denaturing at 94 °C for 15 s, annealing at 66 °C for 15 s, and extension at 72 °C for 15 s, and final extension at 72 °C for 1 min.
For Mycoplasma DNA detection, samples were subjected to 16S rRNA gene amplification using a universal Mycoplasma primer pair (HBT-F-5’ ATA CGG CCC ATA TTC CTA CG-3’ and HBT-R-5’ TGC TCC ACC ACT TGT TCA-3’), 25-50 ng genomic DNA in a total volume of 25 μL, with the following thermal protocol: 95 °C for 15 min and 50 cycles of 95 °C for 10 s, 55 °C for 15 s and 72 °C for 30 s, and 72 °C for 1 min.
Purified PCR amplicons were sequenced using an ABI PRISM 377 Genetic Analyzer (Applied Biosystems, Inc.). Representative sequences of Bartonella (MK953939-MK953940) and Mycoplasma (MK959182) generated from this study were deposited in the National Center for Biotechnology Information GenBank. A neighbour-joining (NJ) phylogenetic tree was plotted using MEGA6 (https://megasoftware.net/). The NJ bootstrap values were estimated using 1 000 replicates with Kimura’s two-parameter model of substitution (K2P distance). Brucella abortus (X95889) and Brucella melitensis (AY513568) were used as outgroups for the construction of Bartonella and Mycoplasma phylogenetic trees, respectively. Based on the 16S-23S fragment of Bartonella, successful PCR amplification was found in 5 out of 134 samples (3.73%). NJ phylogenetic analysis revealed that all five sequences clustered with Bartonella (B.) phoceensis sequence (AY515123) retrieved from the National Center for Biotechnology Information GenBank. B. phoceensis showed a sister relationship with B. rattimassiliensis, and distantly separated from the other Bartonella species [Figure 1]. For Mycoplasma, three samples (2.24%) were positive to 16S rRNA fragment and successfully sequenced. The three sequences formed a monophyletic clade with the sequences of Candidatus Mycoplasma (M.) haemomuris subsp. ratti (AB758434, AB758435 and AB758439) and showed a close relationship with Candidatus M. haemomuris subsp. musculi [Figure 2].
|Figure 1: Neighbour-joining phylogenetic tree of Bartonella spp. based on the 16S-23S intergenic spacer region. Bootstrap values (NJ) are shown on the branches. Newly generated sequences are in bold.|
Click here to view
|Figure 2: Neighbour-joining phylogenetic tree of Mycoplasma spp. based on the 16S rRNA sequences. Bootstrap values (NJ) are shown on the branches. Newly generated sequences are in bold.|
Click here to view
This study provides the first evidence for the presence of B. phoceensis and Candidatus M. haemomuris subsp. ratti in synanthropic rodents in Malaysia. Their prevalence rates (3.73% for Bartonella and 2.24% for Mycoplasma), however, were considered low in comparison to that previously reported in Malaysia. In the earlier study, five different Bartonella species (i.e., B. tribocorum, B. rattimassiliensis, B. coopersplainsensis, B. elizabethae, and B. queenslandensis) (13.7%) were isolated from kidney and spleen homogenates of rats. Nevertheless, the detection protocols adopted (16S-23S versus gltA and rpoB; and blood versus kidney and spleen) may have contributed to the discrepancies in the recovered species in both studies. Among the detected species, B. rattimassiliensis, B. tribocorum, and B. elizabethae were implicated as causing human infections in Thailand. The pathogenicity and zoonotic potential of B. phoceensis are unknown.
Mycoplasma spp. from various animals in Malaysia were reported at varying frequencies such as M. haemofelis (11.7%) in cats, M. wenyonii and Candidatus M. haemobos (69.0%) in cattle, and several species in various animal samples received in a veterinary diagnostic laboratory .
Investigation of Mycoplasma in rodents in Malaysia is underappreciated, though M. arthritidis was reported in three rats (out of 10 rats) in a previous study. In contrast, in the present study, we detected Candidatus M. haemomuris subsp. ratti, a subspecies of M. haemomuris which was recently incriminated as the anaemic pathogen of rats in Japan, and differentiated from Candidatus M. haemomuris subsp. musculi which mainly infects mice. Animal Mycoplasma spp. rarely infect humans, but their zoonotic potential could not be disregarded since cases of human haemoplasma infection have been documented.
In conclusion, we report here the occurrence of B. phoceensis and Candidatus M. haemomuris subsp. ratti in synanthropic rodents for the first time in Malaysia. Our results suggest that synanthropic rodents can serve as the reservoir for these vector-borne pathogens. Nevertheless, their routes of transmission and zoonotic potential require further investigation.
The study protocol [PAR/20/09/2011/J (R)] was reviewed and approved by the Institutional Animal Care and Use Committee, University of Malaya, Malaysia.
Conflict of interest statement
The authors declare that there are no competing interests.
This study was financially supported by the University of Malaya research grant (RP021C-16SUS) and Higher Institution Centre of Excellence (HICoE) program (MO002-2019).
V.L.L. and T.K.T. wrote the manuscript and performed the experiments, J.I., S.A. and Y.A.L.L. contributed to the final version of the manuscript.
| References|| |
Tan TK, Low VL, Ng WH, Ibrahim J, Wang D, Tan CH, et al. Occurrence of zoonotic Cryptosporidium
and Giardia duodenalis
species/genotypes in urban rodents. Parasitol Int
Diniz PP, Maggi RG, Schwartz DS, Cadenas MB, Bradley JM, Hegarty B, et al. Canine bartonellosis:
Serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae
and Bartonella vinsonii
subsp. berkhoffi. Vet Res
Criado-Fornelio A, Martinez-Marcos A, Buling-Saraña A, Barba-Carretero JC. Presence of Mycoplasma haemofelis, Mycoplasma haemominutum
and piroplasmids in cats from southern Europe: A molecular study. Vet Microbiol
Tay ST, Mokhtar AS, Zain SN, Low KC. Isolation and molecular identification of Bartonellae
from wild rats (Rattus
species) in Malaysia. Am J Trop Med Hyg
Kosoy M, Bai Y. Bartonella
bacteria in urban rats: A movement from the jungles of Southeast Asia to metropoles around the globe. Front Ecol Evol
Aklilu E, Shaharulnizim N, Francis JJ, Anurrdin SH. Molecular investigation of Mycoplasma haemofelis
in stray cats in Kota Bharu, Kelantan. Trop Biomed
Mohd Hasan LI, Kho KL, Koh FX, Hassan Nizam QN, Tay ST. Molecular evidence of hemoplasmas in Malaysian cattle and ticks. Trop Biomed
Dahlia H, Tan LJ, Zarrahimah Z, Chandrawathani P, Ramlan M. Isolation of Mycoplasma
species from various animal hosts in Malaysia. Malaysian J Vet Res
Premaalatha B, Nurulaini R, Zawida Z, Norakmar I, Imelda LV, Adnan M, et al. A survey of bacterial and parasitic infections of rats caught in the Veterinary Research Institute (VRI), Ipoh. Malaysian J Vet Res
Harasawa R, Fujita H, Kadosaka T, Ando S, Rikihisa Y. Proposal for ‘Candidatus
Mycoplasma haemomuris subsp. musculi’ in mice, and ‘Candidatus
Mycoplasma haemomuris subsp. ratti’ in rats. Int J Syst Evol Microbiol
[Figure 1], [Figure 2]
|This article has been cited by|
||Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations
| ||Siti Nurul Izzah Mohd-Azami, Shih Keng Loong, Jing Jing Khoo, Nurul Aini Husin, Fang Shiang Lim, Nur Hidayana Mahfodz, Siti Nabilah Ishak, Farah Shafawati Mohd-Taib, Benjamin L. Makepeace, Sazaly AbuBakar |
| ||Tropical Medicine and Infectious Disease. 2023; 8(2): 74 |
|[Pubmed] | [DOI]|
||A novel clade of bat-associated Bartonella detected in the bat fly Leptocyclopodia ferrari (Diptera: Nycteribiidae) parasitizing Cynopterus brachyotis (Chiroptera: Pteropodidae)
| ||Van Lun Low, Tiong Kai Tan, Kamil Azmi Tohiran, Yvonne Ai Lian Lim, Sazaly AbuBakar, Dzulhelmi Muhammad Nasir |
| ||Veterinary Microbiology. 2022; 264: 109284 |
|[Pubmed] | [DOI]|
||Emerging rodent-associated Bartonella: a threat for human health?
| ||Maria Krügel, Nina Król, Volkhard A. J. Kempf, Martin Pfeffer, Anna Obiegala |
| ||Parasites & Vectors. 2022; 15(1) |
|[Pubmed] | [DOI]|