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ORIGINAL ARTICLE
Year : 2020  |  Volume : 13  |  Issue : 12  |  Page : 557-565

Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling


1 Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Republic of Korea
2 Department of Biomaterials Science, Pusan National University, Miryang 50463, Republic of Korea
3 Department of Biology and Chemistry, Changwon National University, Changwon 51140, Republic of Korea
4 Department of Horticultural Bioscience; Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea

Correspondence Address:
Young Whan Choi
Department of Horticultural Bioscience; Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463
Republic of Korea
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Source of Support: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(116027032HD020), Conflict of Interest: None


DOI: 10.4103/1995-7645.296724

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Objective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais. Methods: Dried mulberry leaves, dried mulberry leaves with 50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25 °C, after which the dried mulberry leaves and fermented product were extracted with 70% ethanol and subjected to high performance liquid chromatography (HPLC). The contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid were determined. We then used the 3T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro. Results: HPLC showed that fermentation changed the contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid. Furthermore, fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves, fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption. Additionally, fermented dried mulberry leaves with 50% raw silkworm pupa inhibited PPAR-γ signaling. Conclusions: Fermentation with Cordyceps militaris enhanced anti-adipogenesis efficacy of mulberry leaves.


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