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ORIGINAL ARTICLE
Year : 2020  |  Volume : 13  |  Issue : 11  |  Page : 494-502

Phytochemical profiling and biological activity of Plectranthus amboinicus (Lour.) mediated by various solvent extracts against Aedes aegypti larvae and toxicity evaluation


1 Department of Biotechnology, School of Biosciences, Periyar University, Salem, Tamil Nadu-636 011; Department of Biotechnology, Rathnavel Subramaniam College of Arts and Science, Sulur-641402, Tamil Nadu, India
2 Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul-05006, South Korea
3 Department of Biotechnology, School of Biosciences, Periyar University, Salem, Tamil Nadu-636 011, India
4 Nutritional Improvement of Crops, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
5 Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China
6 Department of Biotechnology, School of Biosciences, Periyar University, Salem, Tamil Nadu-636 011; Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli-620024, Tamil Nadu, India

Correspondence Address:
Balamuralikrishnan Balasubramanian
Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul-05006
South Korea
Perumal Pachiappan
Department of Biotechnology, School of Biosciences, Periyar University, Salem, Tamil Nadu-636 011; Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli-620024, Tamil Nadu
India
Wenchao Liu
Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, Guangdong 524088
China
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Source of Support: This work was partially supported by Innovative Strong School Engineering Youth Talent Project (2017KQNCX090), Conflict of Interest: None


DOI: 10.4103/1995-7645.295360

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Objective: To analyze the phytochemical compounds and to investigate the bio-toxic efficacy of various solvent extracts of Plectranthus amboinicus against mosquito larvae activity and lethality on non-targeting organisms. Methods: The methanol, ethyl acetate, hexane, and aqueous extracts of Plectranthus amboinicus were subjected to analyze the mosquitocidal activity against the dengue vector, Aedes aegypti and toxicity assays on zebra fish and brine shrimp. Three replications were performed, and negative control was also maintained. Amongst, ethyl acetate extract of Plectranthus amboinicus was chosen for the determination of bio-active compounds. Results: The mosquitocidal assays of methanol and ethyl acetate extracts of Plectranthus amboinicus showed the maximal activity with minimal concentration against the 4th instar mosquito-larvae of Aedes aegypti through the following lethal concentration (LC50 and LC90) values: 53.36 & 92.51 μg/mL and 13.64 & 86.09 μg/mL, respectively. In addition, the plant extracts showed no toxicity on zebra fish embryo and brine shrimp assays. The gas-chromatography analysis of the ethyl acetate extract of Plectranthus amboinicus revealed the presence of seven different compounds. Among them, PAEA-fraction 60 contained a major active bioactive compound, hexadecanoic acid, methyl ester (270.0). Conclusions: Plectranthus amboinicus possesses mosquitocidal properties and could be used as a potential alternative source for preparing the mosquitocidal agents.


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