| ORIGINAL ARTICLE |
|
| Year : 2019 | Volume
: 12
| Issue : 3 | Page : 106-116 |
|
|
Antihydatic and immunomodulatory effects of Algerian propolis ethanolic extract: In vitro and in vivo study
Nahla Deghbar1, Dalila Mezioug1, Touri Kahina1, Yacine-Miloud Medjdoub2, Chafia Touil- Boukoffa1
1 Cytokines and NO Synthases-Immunity and Pathogeny team, Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria
2 Department of Thoracic Surgery, Mustapha Pacha Hospital, Algiers, Algeria
Correspondence Address:
Dalila Mezioug
Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, PB 32, El-Alia, 16111, Algiers
Algeria
 Source of Support: None, Conflict of Interest: None  | 6 |
DOI: 10.4103/1995-7645.254936
|
|
|
Objective: To evaluate the in vitro and in vivo effect of the Algerian propolis ethanolic extract (EEP) against Echinococcus granulosus (E. granulosus) infection.
Methods: In vitro scolicidal activity of EEP was investigated on the protoscolices of hydatid cyst. This in vitro study was conducted by using an in vivo assay. BALB/c mice were inoculated with E. granulosus and treated with propolis for three months. Hydatid cysts development was assessed. Nitric oxide (NO), tumor necrosis factor alpha (TNF-α) production and inducible NO synthase, NF-κB, and TNF-α spleen expression were estimated by Griess method and immunofluorescence respectively.
Results: Our study revealed that EEP has a high scolicidal activity against E. granulosus. Oral administration of EEP decreased TNF-α, NF-κB and inducible NO synthase expression in the spleen tissues in the CE+EEP group, in comparison with the CE group. Concomitantly, EEP treatment caused an important systemic decrease in NO and TNF-α levels. These findings are associated with the reduction of CE development.
Conclusions: This is the first report demonstrating with interest the antihydatic and immunomodulatory effects of the Algerian EEP, suggesting its therapeutic potential for the hydatid disease treatment.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
