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ORIGINAL ARTICLE
Year : 2019  |  Volume : 12  |  Issue : 1  |  Page : 14-18

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus


Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709, Korea

Correspondence Address:
Young-Seob Lee
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709
Korea
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1995-7645.250339

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Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/ blaR1 gene expressions.


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