Impact Factor 2021: 3.041 (@Clarivate Analytics)
5-Year Impact Factor: 2.776 (@Clarivate Analytics)
Impact Factor Rank: 10/24, Q2 (Tropical Medicine)
  • Users Online: 75
  • Print this page
  • Email this page
Year : 2018  |  Volume : 11  |  Issue : 3  |  Page : 260-264

Steroidal alkaloids ameliorate cell proliferation, oxidative stress, inflammation and histology outcome in vitro and in vivo

1 West School of Pharmacy, Sichuan University, Chengdu 610041; Medical School of Hubei University for Nationalities; Huhei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Enshi 445000, China
2 West School of Pharmacy, Sichuan University, Chengdu 610041, China

Correspondence Address:
Shu Wang
West China School of Pharmacy, Sichuan University, No.17 People’s South Road, Chengdu 610041
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1995-7645.228444

Rights and Permissions

Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-α-induced human RA-FLS MH7A in vitro, and against complete Freund’s adjuvant (CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group (CFA), steroidal alkaloids (STA) groups (5.0, 2.5 and 1.25 g/kg BW), and sarcovagine D (SD) groups (50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7A cell culture proliferation and downregulated NO levels, and inflammatory cytokines (IL-1ß, IL-6 and PGE2) in vitro. The rats treated with CFA induced ankle inflammation and lymphocyte infiltration, upregulated IL-1ß, TNF-α, IL-6, PGE2, MDA and NO levels, and downregulated IL-10 and SOD. On the contrary, steroidal alkaloids ameliorated all the changes induced by CFA in rats, suggesting the anti-inflammatory effect of steroidal alkaloids and sarcovagine D. Conclusions: The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded361    
    Comments [Add]    
    Cited by others 1    

Recommend this journal