Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells

You-Wei Huang; Jia-Hong Chen; Zi-Xi Qin; Jie-Kun Chen; Ren-Dong Hu; Zheng Wu; Xi Lin

doi:10.4103/1995-7645.228440

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ORIGINAL ARTICLE

Year : 2018 | Volume : 11 | Issue : 3 | Page : 240-244

Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells

You-Wei Huang¹, Jia-Hong Chen¹, Zi-Xi Qin¹, Jie-Kun Chen¹, Ren-Dong Hu¹, Zheng Wu², Xi Lin¹
¹ Department of Pharmacology, Medical College, Jinan University, Guangzhou, China
² Key laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou; Department of Developmental and Regenerative Biology, Jinan University, Guangzhou, China

Correspondence Address:
Xi Lin
Department of Pharmacology, Medical College, Jinan University, No.601, West Huangpu Avenue, Tianhe District, Guangzhou, Guangdong
China
Zheng Wu
Key laboratory for Regenerative Medicine, Ministry of Education, Jinan University, No.601, West Huangpu Avenue, Tianhe District, Guangzhou, Guangdong
China

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1995-7645.228440

Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS. Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.

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