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Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells
Na Cheng1, Xiao-Ran Liu2, Da-Wei Liu3, Fang Liu4, Jin Xiang5, Di Yang6, Guo-Qiang Zhao7
1 Department of Pathology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
2 Emergency Department, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, China
3 Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
4 Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
5 Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong Province, China
6 Department of Pathology, Nanhai District People’s Hospital, Foshan 528200, Guangdong Province, China
7 Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province; Pathology Teaching and Research Section, Sun Yat-sen University Zhongshan School of Medicine, Guangzhou 510080, Guangdong Province, China
Correspondence Address:
Guo-Qiang Zhao
Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan Road, Guangzhou 510080, Guangdong Province, China; Pathology Teaching and Research Section, Sun Yat-sen University Zhongshan School of Medicine; No. 58 Zhongshan Road, Guangzhou 510080, Guangdong Province
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/1995-7645.228439
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Objective: To study the effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells. Methods: Regenerated tissue extracts after liver injury were used to induce SK-HEP1 cells after enrichment, their effects on the proliferation, differentiation, migration and invasion of SK-HEP1 cells were observed through in vitro cell culture, MTT, flow cytometry and transwell assays. Results: In response to the action of regenerated tissue extracts after liver injury, SK-HEP1 cells were blocked in G0/G1 phase, their growth rate was distinctly reduced. The number of SK-HEP1- fj colonies decreased. The migration ability of SK-HEP1 cells showed a decreased trend on day 7 and day 11 after induction. SK-HEPl’s invasion ability clearly decreased on days 7 and 11 after induction, especially on day 7. Conclusions: To a certain extent, regenerated tissue extracts after liver injury can inhibit the proliferation, differentiation, migration and invasion of hepatoma cells, showing an important potential of being a differentiating agent for the treatment of liver cancer.
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