Figure 1: Representative light microphotographs from the cerebral cortex of mice (H & E, × 400). (A) Vehicle: normal neurons (N). (B) Rotenone: shrunken cytoplasm, numerous dark pyknotic (arrow) and apoptotic (arrowhead) nuclei in neu rons, surrounded by perineuronal vacuolation (V) with congested capillaries (R) and focal gliosis. (C) Rotenone + misoprostol 10 μ g/kg: still pyknotic (arrow) and apoptotic (arrowhead) nuclei, acidophilic cytoplasm, vacuolation of neuropil (V) and congested capillaries (R). (D) Rotenone + misoprostol 100 μ g/kg: few pyknotic (arrow) and apoptotic nuclei (arrowhead) with vacuolation (V). (E) Rotenone + misoprostol 1 000 μ g/kg: almost normal tissue with very few pyknotic (arrow) and apoptotic nuclei in neurons (arrowhead).