Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 4  |  Page : 163--169

Characterization of the salivary microbiome in healthy Thai children


Izumi Mashima1, Citra F Theodorea2, Boonyanit Thaweboon3, Sroisiri Thaweboon3, Tippanart Vichayanrat4, Frank A Scannapieco5, Futoshi Nakazawa6 
1 Department of Microbiology, School of Pharmacy, Aichi Gakuin University, Japan
2 Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Indonesia
3 Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Thailand
4 Department of Community Dentistry, Faculty of Dentistry, Mihidol University, Thailand
5 Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, USA
6 Department of Microbiology, School of Dentistry, Health Sciences University of Hokkaido, Japan

Correspondence Address:
Futoshi Nakazawa
1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293
Japan

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r2=0.887 to r2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups (P<0.05). Furthermore, the proportion of Veillonella parvula, as well as Rothia aeria and Rothia dentocariosa tended to increase with worse oral hygiene status, which was also related to higher dental caries history. Conclusions: The differences in the salivary microbiome as related to geographic regions suggest that environmental factors, which may include dietary habits, could influence the predominant bacteria found in the mouth of Thai children, especially the genus Veillonella and Prevotella. The ratio of Veillonella parvula, Rothia aeria and Rothia dentocariosa may be indicators of worse oral hygiene status and future caries in this population.


How to cite this article:
Mashima I, Theodorea CF, Thaweboon B, Thaweboon S, Vichayanrat T, Scannapieco FA, Nakazawa F. Characterization of the salivary microbiome in healthy Thai children.Asian Pac J Trop Med 2019;12:163-169


How to cite this URL:
Mashima I, Theodorea CF, Thaweboon B, Thaweboon S, Vichayanrat T, Scannapieco FA, Nakazawa F. Characterization of the salivary microbiome in healthy Thai children. Asian Pac J Trop Med [serial online] 2019 [cited 2020 Jul 7 ];12:163-169
Available from: http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=4;spage=163;epage=169;aulast=Mashima;type=0