Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 2  |  Page : 151--154

Effect of iron overload on electrophysiology of slow reaction autorhythmic cells of left ventricular outflow tract in guinea pigs


Ling Fan1, Li-Feng Chen2, Jing Fan3, Lan-Ping Zhao2, Xiao-Yun Zhang2 
1 Department of haematology, the First Affiliated Hospital of Hebei North University, 075000 Zhangjiakou, Hebei, China
2 Department of Physiology, Basic Medical College, Hebei North University, 075000 Zhangjiakou, Hebei, China
3 Department of Obstetrics and Gynecology, the Sixth Hospital of Zhangjiakou, 075000 Zhangjiakou, Hebei, China

Correspondence Address:
Li-Feng Chen
Department of Physiology, Basic medical college, Hebei North University, 075000 Zhangjiakou, Heibei
China

Objective: To investigate the electrophysiology effects and mechanism of iron overload on the slow response autorhythmic cells in the left ventricular outflow tract of guinea pigs. Methods: Standard microelectrode cell recording techniques were adopted to observe the electrophysiological effects of different concentrations of Fe2+ (100 μmol/L, 200 μmol/L) on the left ventricular outflow tract autorhythmic cells. Heart tissues were perfused with FeSO4 (200 μmol/L) combing with CaC12 (4.2 mmol/L), Verapamil, (1 μmol/L), and nickel chloride (200μmol/L) respectively to observe the influences of these contents on electrophysiology of FeSO4 (200 μmol/L) on the left ventricular outflow tract autorhythmic cells. Results: Fe2+ at both 100 μmol/L and 200 μmol/L could change the electrophysiological parameters of the slow response autorhythmic cells of the left ventricular outflow tract in a concentration-dependent manner resulting into decrease in Vmax, APA and MDP, slower RPF and VDD, and prolonged APD50 and APD90 (P all <0.05). Besides, perfusion of increased Ca2+ concentration could partially offset the electrophysiological effects of Fe2+ (200 μmol/L). The L-type calcium channel (LTCC) blocker Verapamil (1 μmol/L) could block the electrophysiological effects of Fe2+ (200 μmol/L). But the T-type calcium channel (TTCC) blocker nickel chloride (NiCl2, 200 μmol/L) could not block the electrophysiological effects of Fe2+ (200 μmol/L). Conclusions: Fe2+ can directly change the electrophysiological characteristics of the slow response autorhythmic cells of the left ventricular outflow tract probably through the L-type calcium channel.


How to cite this article:
Fan L, Chen LF, Fan J, Zhao LP, Zhang XY. Effect of iron overload on electrophysiology of slow reaction autorhythmic cells of left ventricular outflow tract in guinea pigs.Asian Pac J Trop Med 2018;11:151-154


How to cite this URL:
Fan L, Chen LF, Fan J, Zhao LP, Zhang XY. Effect of iron overload on electrophysiology of slow reaction autorhythmic cells of left ventricular outflow tract in guinea pigs. Asian Pac J Trop Med [serial online] 2018 [cited 2019 Dec 13 ];11:151-154
Available from: http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=151;epage=154;aulast=Fan;type=0