Impact Factor 2018: 1.772 (@Clarivate Analytics)
5-Year Impact Factor: 1.772 (@Clarivate Analytics)
  • Users Online: 151
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2019  |  Volume : 12  |  Issue : 10  |  Page : 457-462

Detection of Trypanosoma spp. in Bandicota indica from the Thai-Myanmar border area, Mae Sot District Tak Province, Thailand


1 Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
2 Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand

Correspondence Address:
Phuangphet Waree Molee
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok
Thailand
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1995-7645.269907

Get Permissions

Objective: To investigate the prevalence of trypanosome infection and their phylogeny in Bandicota indica rats from the cadmium-contaminated area of Mae Sot and the Myanmar border. Methods: Blood samples were taken from 100 animals, and parasite infection was examined by light microscopy observation and polymerase chain reaction (PCR) studies. Results: Trypanosoma spp. infection was found in 20% of the thin blood smear samples. PCR showed positive 623 bp DNA bands in 21 samples (21%). The sequencing analysis showed that all of the samples (100%) had the Trypanasoma lewisi 18S ribosomal RNA gene. Phylogenetic analysis confirmed that these 16 isolates of Trypanosoma spp. were closely related to Trypanasoma lewisi. Conclusions: Molecular detection using PCR is as effective as conventional light microscopy analysis. This study confirms that trypanosomal infection in rodents is still high; therefore, fleas as their vectors need to be controlled in order to prevent transmission to humans.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed185    
    Printed14    
    Emailed0    
    PDF Downloaded83    
    Comments [Add]    

Recommend this journal