Impact Factor 2017: 1.634 (@Clarivate Analytics)
5-Year Impact Factor: 1.677 (@Clarivate Analytics)
  • Users Online: 109
  • Print this page
  • Email this page


 
 
Table of Contents
REVIEW ARTICLE
Year : 2018  |  Volume : 11  |  Issue : 11  |  Page : 597-608

Brucellosis: Pathophysiology and new promising treatments with medicinal plants and natural antioxidants


1 Razi Herbal Medicines Research Center; Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
5 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Date of Submission22-Jul-2018
Date of Decision10-Nov-2018
Date of Acceptance21-Nov-2018
Date of Web Publication30-Nov-2018

Correspondence Address:
Mahmoud Rafieian-Kopaei
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1995-7645.246336

Get Permissions

  Abstract 


Brucellosis is an old, infectious and common zoonosis whose causative agents are Gramnegative bacteria from the Brucella genus. Brucellosis is transmitted through direct contact with infected animals or using unpasteurized dairy products of goats, pigs, camels, sheep, buffalo and cows. Brucellosis is still the most common zoonosis in the world, with most of cases occurring in developing countries. Today, an approach to traditional medicine and medicinal plants, especially with regards to the repeated recommendations of the World Health Organization, is a necessity. One-third of chemical drugs are produced by using plants and there is a high potential to produce more drugs from plants. Medicinal plants are helpful in the management of various conditions, especially bacterial diseases. Although there is not enough scientific evidence regarding the clinical effectiveness of herbal drugs for the treatment of brucellosis, there is strong evidence on the antimicrobial effects of herbal drugs to prevent infection. Therefore, this article seeks to describe the antibacterial effects of some plant-derived essential oils or extracts, so that they can serve as promising choices to develop new anti-Brucella medications, as suitable alternatives to conventional antibiotics for brucellosis, as much as possible, taking into account the benefits of these herbal drugs.

Keywords: Brucellosis, Undulant fever, Malta fever, Herbal drugs, Phytotherapy, Antioxidant


How to cite this article:
Alizadeh M, Safarzadeh A, Bahmani M, Beyranvand F, Mohammadi M, Azarbaijani K, Rafieian-Kopaei M, Abbaszadeh S. Brucellosis: Pathophysiology and new promising treatments with medicinal plants and natural antioxidants. Asian Pac J Trop Med 2018;11:597-608

How to cite this URL:
Alizadeh M, Safarzadeh A, Bahmani M, Beyranvand F, Mohammadi M, Azarbaijani K, Rafieian-Kopaei M, Abbaszadeh S. Brucellosis: Pathophysiology and new promising treatments with medicinal plants and natural antioxidants. Asian Pac J Trop Med [serial online] 2018 [cited 2018 Dec 16];11:597-608. Available from: http://www.apjtm.org/text.asp?2018/11/11/597/246336




  1. Introduction Top


Brucellosis is an old, infectious and common zoonosis whose causative agents are Gram-negative bacteria from the Brucella genus. Brucellosis is transmitted through direct contact with infected animals or using unpasteurized dairy products of goats, pigs, camels, sheep, buffalo and cows. People working in slaughterhouses or veterinarians are at high risk of developing the disease. Brucellosis is more common in children than in adults[1],[2] Non-specific symptoms of brucellosis include fever, chills, restlessness, headache, fatigue and weakness[3],[4],[5]. One of the most common symptoms of this disease is bone involvement that is seen in over half of the affected people[6]. The association between Brucella and brucellosis was first discovered by an army surgeon named David Bruce. He isolated bacteria from the spleen of patients in Malta from 1886 to 1887. Brucellosis is still one of the most common zoonosis in the world and more than 500 000 cases each year are diagnosed with the disease, most of whom live in developing countries. The number of brucellosis cases are over 10 000 000 people in some countries where brucellosis is endemic[7],[8]. Centers for Disease Control and Prevention has classified brucellosis as a category B bioterrorism agent due to its easy transmission[9].

The disease cycle can be acute, sub-acute or chronic[10]. Four different Brucella species, i.e., Brucella suis (B. suis), Brucella melitensis (B. melitensis), Brucella abortus (B. abortus) and Brucella canis can be pathogenic to humans[11]. Among these species, B. melitensis is the most contagious species, followed by B. abortus and B. suis[9]. Brucella species are intracellular, obligate and Gramnegative coccobacilli and are not able to produce spores[12]. Brucella species have no classic virulence genes encoding capsules, plasmids, pili or exotoxins, and because of factors that cause the bacteria to acquire resistance to phagocytes and increase the proliferation of phagocytic cells, they are relatively less known than other pathogenic bacteria[13].

The O-polysaccharide chain on the lipopolysaccharide layer plays an important role in pathogenicity of the bacteria. The O-polysaccharide chain of Brucella prevents complement accumulation on bacterial surfaces[14],[15]. This is done by preventing the C1q access to the external surface of the targeted proteins; and a study led to the hypothesis that the size of the O chain acts as a protective factor against the complement system[16]. Brucella lipopolysaccharide protects the bacteria against antibacterial cationic peptides in addition to blocking the pathway for activation of the complement. It has been shown that brucellosis is resistant to a large variety of peptides such as defensin NP-2, lactoferrin, cecropines lysozyme, bactenecin-derived peptides, defensin-like antibiotic polymyxin B, as well as lysosomal compounds produced from polymorphonuclear leukocytes[17],[18]. By controlling the adenine and guanine monophosphate systems, the bacteria prevent the integration of phagolysosomes, the release of myeloproxidase, and production of tumor necrosis factor (TNF), and thus cause the disease[19]. Brucella can survive inside certain macrophages in certain ways. It protects itself against the host immune system in certain ways such as stopping macrophage apoptosis, suppressing T1 specific immune responses and inhibiting TNF-α production[20],[21],[22]. Brucella should prevent Th1 polarization in order to protect itself against the activation of macrophages and the production of cytotoxic T cells. Polarization of the immune system is accomplished by cytokines that are rapidly produced by infected macrophages. Therefore, stopping the expression of cytokines such as TNF is one of the methods of the pathogens to fight the immune system[20],[23]. Investigation of B. suis-infected monocytes revealed that the expression of the Al gene, which is one of the genes of the bcl-2 family, increases in these cells, and contributes to the survival of the cells and, consequently, resistance to apoptosis. B. suis-infected cells are also resistant to the signaling of Fas ligand or apoptosis-inducing interferon gamma, which protects the host cell against different cytotoxic processes of the immune system[17].

Brucellosis is a systemic disease with a wide range of clinical symptoms so that it can be asymptomatic or cause life-threatening symptoms[24],[25]. Symptoms include headache, fever, sweating, weight loss and back pain; and the most commonly observed symptoms due to the disease are meningitis, dizziness, hepatomegaly, hypoesthesia and splenomegaly[26]. Due to the specific characteristics of the bacterium, Brucella is one of the most important causes of fever of unknown origin[27],[28]. This disease can involve various organs in the body. Bone involvement is the most commonly observed involvement in half of the people with brucellosis[29],[30]. The mortality rate from brucellosis is less than 1% and the most common cause of death is heart involvement. Brucellosis also causes certain disorders such as endocarditis, pericarditis, myocarditis, and inflammation of the walls of the veins and arteries[31],[32].

Nervous system disorders occur in 10% of patients and can lead to meningoencephalitis, myelitis, cerebellar involvement, cranial nerve palsy, stroke, spinal cord atrophy, chronic and acute meningitis, polyradiculoneuropathy and paraplegia. Neurological disorders, especially in the white matter, are due to immune responses to bacteria[33],[34],[35]. It can also affect the genitourinary system. Genital tract infections include testicular and epididymal inflammation, renal and testicular abscesses, prostate inflammation, glomerulonephritis and calcified nodules, which are observed in 2%-20% of the patients. The cause of epididymal inflammation is the presence of various sugars similar to erythritol in the epididymis, because bacteria need this sugar for growth[36],[37]. In addition to the aforementioned disorders, this disease also causes a lot of hematologic disorders, including lymphocytosis, leukopenia, leukocytosis, thrombocytopenia, increased erythrocyte sedimentation rate and anemia; hematologic disorders are caused by hemophagocytosis, microgranuloma and hyperplasm[38]. Liver is another organ that can be involved in brucellosis. Hepatomegaly and splenomegaly have been reported in 63% and 56% of patients, respectively. Cirrhosis of the liver, hepatic parenchymal necrosis and hyperplasia of Kupffer cells are also some of the studied liver involvements[39],[40].

Each year more than 500 000 people acquire brucellosis and most of them live in the Mediterranean region, the west and central Asia, certain parts of Africa and Latin America[4], as well as some Asian countries including Syria, Iraq, Iran[41] and Mongolia[42]. The overall prevalence has been reported to be high but the prevalence in China and South Korea is low[43]. However, the disease remains endemic in some areas of the two countries[44]. Brucellosis is more common in men due to their greater activity than women and children[45]. Brucellosis is an important disease in the Sub Saharan countries and has been reported in almost all African countries[46].

There is strong evidence on the antimicrobial effects of herbal drugs to prevent infection. Furthermore, oxidative stress and inflammation are involved in a lot of diseases[47],[48],[49]. Hence, medicinal plants which usually have antioxidant activity, should have more benefits in infectious diseases which mostly are associated with inflammation. This article seeks to introduce the antibacterial effects of some plant-derived essential oils or extracts, so that they can serve as promising choices to develop new anti-Brucella medications, as suitable alternatives to conventional antibiotics for brucellosis, as much as possible, taking into account the benefits of these herbal drugs.


  2. Retrieve methods Top


To do this systematic review, the databases such as Web of Science, PubMed, PubMed Central, Scopus, Science Direct and Google Scholar were searched for articles on etiology, pathology and epidemiology of brucellosis, as well as the role of herbal drugs in treating undulant fever without publication time constraints. Words or keywords that were used separately in the title/keywords/abstract to retrieve articles were medicinal plant, herb, herbal medicine, traditional Chinese medicine, traditional medicine, natural medicine, phytochemical, herbal drugs and brucellosis, Malta fever, brucellosis etiology, brucellosis epidemiology and brucellosis pathology. Retrieved articles were once again analyzed. The abstracts were examined for the pre-determined inclusion and exclusion criteria. Articles without English abstract and full text were excluded from the analysis. Only the articles that mainly addressed the effect of medicinal plants and their derivatives on the control of the Brucella were analyzed.

A total of 155 articles were retrieved articles from databases. After analysis, 28 articles were deleted for the following reasons: repeatability and old age of publication of articles (16), lack of summaries (5) and lack of access to their full text (7). In the end, 127 articles directly affect the effect of herbs and their derivatives on the control of Brucella bacteria were selected for the study of cytological overview.


  3. Plants used in treating brucellosis Top


The use of medicinal plants has increased among adults and children in recent years, so that approximately 4 per every 10 Americans use alternative therapies such as medicinal plants[50]. More than one-third of chemical drugs are produced from plants and there is a high potential to produce more effective drugs from them. Medicinal plants are used to treat various diseases such as rheumatic disorders, acquired immune deficiency syndrome, cancer, depression and bacterial diseases[51], [52]. Due to low resistance to high temperatures and the carcinogenicity of some of the synthetic compounds, today, attention has been drawn to the use of natural antioxidants. Hence, a list of the most important medicinal plants which are effective against Brucella and have the potential to use as anti-Brucella remedies are presented [Table 1].
Table 1: Plants and their most important active ingredients that are potentially effective to prevent undulant fever.

Click here to view


3.1. Alhagi camelorum (A. camelorum)

A. camelorum is a perennial shrub with a relatively deep creeping root and is used as a traditional drug for diuresis or to remove kidney stones and pain[53]. The aqueous extract of the plant at 500 mg/mL caused an minimum inhibitory concentration (MIC) of 27 mm within 48 h under 37 °C[54].

3.2. Allium sativum (A. sativum)

A. sativum grows around the world depending on seasonal changes, however, its production is limited by the region and the type of weather. The plant is cultivated in autumn and is harvested in the summer[55]. Shapoury et al. reported the effect of chloroform A. sativum extract on B. melitensis (Rev1) and B. abortus (S19). In that study, chloroform A. sativum extract at dilutions of 1:10 to 1:160 could inhibit the growth of B. melitensis (Rev1) and B. abortus (S19) at 37 °C within 3 d. It was also found that the A. sativum extract could inhibit both Brucella species at 4 °C and 37 °C after 2 h growth[56].
Figure 1: The criteria and the number of entry and exit articles.

Click here to view


3.3. Arctium lappa (A. lappa)

A. lappa, a herbaceous plant with large leaves and long root, is used orally as a medicine. In a study, the effect of A. lappa extracts on B. melitensis 16 M within 72 h was investigated. The aqueous extract of the plant at 544 mg/mL at dilutions of 1, 1:2, 1:04, 1:08, 1:16 and 1:32 caused no growth inhibition zone, but the ethanol extract of its leaf at 101.875 mg/mL and dilutions of 1, 1:2, 1:04, 1:08 and 1:16 could cause growth inhibition zones of 27.5, 16.0, 14.0, 12.0 and 4.0 mm, respectively. While the acetone extract of its leaf at 109.5 mg/mL in dilutions of 1, 1:2 and 1:4, caused growth inhibition zones of 15.5, 12.0 and 8.0 mm, respectively. Aqueous A. lappa extract at 259 mg/mL had no effect on inhibition of Brucella, but the ethanol extract of the plant at 116.25 mg/mL in dilutions of 1, 1:2, 1:04 and 1:08 caused growth inhibition zones of 24.5, 18.0, 12.0 and 10.0 mm, and the acetone extract at 200 mg/mL in dilutions of 1: 1: 2, 1: 4 and 1:8 caused the growth inhibition zones of 18.0, 12.5, 11.0 and 10 mm[57].

3.4. Berberis integerrima

Berberis integerrima is a deciduous shrub that reaches a height of 5 m, has egg-shaped leaves, yellow flowers and bark and red fruits, growing in the Middle East and central Asia[58]. In a study, the effect of the active ingredients of Berberis integerrima on B. abortus (RB-51) after 17 h incubation at 37 °C was investigated. Finally, the MICs of the compounds palmatine, berberine, columbamine and jatrorrhizine at 620, 500, 250 and 120 μg/mL were 6.25, 1.56, 3.12 and 0.78 mm, respectively[59].

3.5. Caraway (Carum carvi L.)

Carum carvi is an andromonoecious plant that occurs in many countries in Europe, Asia and Africa, and is traditionally used to produce oil and also is used for pharmaceutical purposes[60]. The essential oil of this plant at 0.3-2 mg/mL has been reported to inhibit the B. abortus isolated from the cattle[61].

3.6. Citrullus colocynthis

Citrullus colocynthis is a desert and perennial plant that spreads in African and Arab countries and India. The fruits of this plant are fleshy with dark green spots and usually yellow when they are ripened. The ethanol extract of Citrullus colocynthis at 25 mg/mL was able to cause a growth inhibition zone of 15 mm in diameter for 24 h under (35 ± 2) °C[62].

3.7. Eucalyptus globulus (E. globulus)

E. globulus is a evergreen tree native to Australia that is also widely found in Spain, Portugal, Italy and India, and is used in traditional medicine for the treatment of common infections[63]. In a study, the effects of ethanol, aqueous and acetone extracts of E. globulus leaf on B. melitensis M16 and B. abortus S99 were examined for 5 d at 37 °C. All extracts of the plant in a dilution of 1: 5 (for the acetone extract at 166 mg/mL and for the ethanol extract at 162.4 mg/mL) caused growth inhibition zones of 25, 30 and 30 mm in diameter and the aqueous extract in a dilution of 1: 320 produced no effect, but the ethanol and acetone extracts caused growth inhibition zones of 15 and 10 mm in diameter for the 16 M strain.

The aqueous, ethanol and acetone extracts of this plant at 1: 5 dilution caused growth inhibition zones of 22.5, 27.5 and 27.5 mm and the ethanol and acetone extracts at the dilution 1: 320 growth inhibition zones of 10 and 14 mm for B. abortus S99, but its aqueous extract at this dilution was ineffective. The aqueous extract of this plant at a dilution of 1:40 (21.62 μg/mL) caused a growth inhibition zone of 20 and 17 mm for the 16M and S99 species. Ethanol extract of E. globulus produced the greatest effect on both Brucella strains followed by the acetone and aqueous extracts[64].

3.8. Humulus lupulus (H. lupulus)

H. lupulus grows in the northern hemisphere, with its best growing conditions in moderate humid climates. H. lupulus has crossed leaves with leaf-stalk up to 7-12 cm in size. Male and female flowers of this plant grow on separate plants and the antimicrobial properties of the plant are mainly due to its acids[65]. Shapouri et al. have shown that B. abortus and B. melitensis were more susceptible to the flower or cone extract of H. lupulus. Various forms of the H. lupulus extract (aqueous, ethanol and acetone) have had more than 3 mm of inhibition zone in dilutions of 1:10 to 1: 320 for both species of Brucella and the ethanol extract had the greatest effect. The aqueous extract of H. lupulus in dilutions of 1:10 to 1: 640 affected B. abortus and B. melitensis within 24 hours, while the acetone and ethanol extracts of the plant have been found to affect in dilutions of 1:10 to 1: 1 280 within 24 h[66].

3.9. Juniperus oxycedrus L.

The MIC of the methanol extract of Juniperus oxycedrus leaf at 300 μg/disc was determined to be 8-13 mm at 37 °C within 24 h, indicating a strong anti-Brucella effect relative to the MIC of 12 mm determined for the antibiotic sulbactam used as a control. However, the aqueous Juniperus oxycedrus extract was ineffective[67].

3.10. Mentha piperita, Origanum majorana, Myristica fragrans, Cinnamomum verum and Citrus limon (C. limon)

In a study, the effects of different plants such as Mentha piperita, Origanum majorana, Myristica fragrans, Cinnamomum verum and C. limon on B. abortus 544 were investigated. The inhibitory effect of 1% essential oil of the bark of Cinnamomum verum persisted after 24-144 h following infection[68]. This plant is an evergreen plant belonging to the Lauraceae family, has anti-allergic and antipyretic properties, and grows in the tropical regions of Africa and Asia[69]. Mentha piperita is a perennial plant and native to Europe, northern America and Canada, has a variety of colors, with fragrant leaves and can grow up to 30-90 cm[70]. It was observed to reduce bacterial growth within 4-144 h. C. limon is a tree of 3-6 m, and its 1% essential oil is able to inhibit bacterial growth within 48-96 hours[68]. Origanum majorana is a perennial and resistant plant that grows wildly in Egypt, eastern Mediterranean region, and northern Europe with mulching materials. The 1% essential oil of its leaf, along with 0.1% essential oil of cinnamon, was able to reduce bacterial growth within 24-144 h[71]. The 1% essential oil of Myristica fragrans, an evergreen that is native to tropical region such as Indonesia, along with C. limon, produces a synergistic effect to inhibit B. abortus 544[68].

3.11. Lavandula pubescens (L. pubescens)

In a study, the effect of raw extracts of L. pubescens aerial parts, which has almost oval leaves and is found mostly in the Middle East, on B. abortus (CMCC 210101) was studied. The MICs of the ultrasonic-microwave extract of L. pubescens at concentrations of 10 and 20 mg/mL were (4.11±0.30) and (7.12 ± 0.40) mm, respectively. The MICs of the ultrasonic-homogenizer extract of the plant at concentrations of 10 and 20 mg/mL were (11.3 ± 0.3) and (12.3 ± 0.4) mm, respectively; and finally, the MICs of the maceration extracts at concentrations of 10 and 20 mg/mL were (10.9 ± 0.4) and (11.9 ± 0.5) mm at 37 °C within 22 h[72].

3.12. Nepeta cataria (N. cataria)

N. cataria is a perennial plant with white to pink flowers and spreads in the eastern Mediterranean regions, western Himalayas, central Asia, southern Siberia and China. The essential oil of N. cataria at 10 μL caused a 10 mm growth inhibition zone diameter within 24 h under 37 °C[73].

3.13. Nigella sativa (N. sativa)

N. sativa is from the Ranunculaceae family and is found in abundance in the Mediterranean region and southern parts of Asia, India, Egypt and Syria, and grows up to 20-30 cm. It has large fruits, each containing 3-7 follicles that contain a large number of seeds[74]. Due to the ability of Brucella to grow at low temperatures, Alsawaf et al. examined the growth rate of the bacteria in the presence of

N. sativa in white cheese in freezer [(2 ± 5) °C]. In that study, N. sativa oil and seed were found to be able to inhibit B. melitensis, and the oil produced a more potent effect. After two days, cheese with 1% of seed (1% of the total cheese content), cheese with 0.3% oil (percentage of total cheese content) were able to reduce B. melitensis growth[75].

3.14. Oliveria decumbens

Oliveria decumbens occurs in south and southwest Iran, is endemic in Iran and is traditionally used to treat diarrhea and fever[76]. The MICs of the ethanol and methanol extracts of this plant at 50, 100, 200 and 400 mg/mL were 8, 10, 13 and 16 mm and 9, 12, 14 and 17 mm, respectively[71].

3.15. Origanum acutidens (O. acutidens)

O. acutidens that grows mostly in calcareous and non-calcareous stones at an altitude of 1 000-3 000 m is an important genus of the Lamiaceae family, includes about 900 species and spreads across the globe. Sökme et al. evaluated the effect of this plant on B. abortus A77. The essential oil of this plant was highly able to eliminate B. abortus (A77), and an MIC of 250 mm was recorded for 10 μL of its extract that was approximately four times higher than the MIC of the antibiotic sulbactam (62.50 mm)[77].

3.16. Origanum syriacum (O. syriacum) and Thymus syriacus (T. syriacus)

T. syriacus is used as a herbal tea and spice, and to treat cough and respiratory disorders, and grows in the eastern Mediterranean region[78],[79]. O. syriacum can grow up to 90 cm in vast regions of the eastern Mediterranean region, southern Turkey, Syria and Cyprus, and is used in Syria to treat respiratory and digestive disorders[79]. The essential oil of T. syriacus aerial parts at 50 mg/mL caused a moderate growth inhibition zone (22.6 mm in diameter), while the O. syriacum essential oil was able to cause a growth inhibition zone of 16.2 mm in diameter[80].

3.17. Peganum harmala L.

Peganum harmala is a perennial and flowering bush, and grows up to 30-100 cm. The plant spreads in northern Africa, the Mediterranean region, the Middle East, Pakistan, India and Iran[81]. The effects of the extracts of the seed, leaf, flower and root of the plant within 24 h under 37 °C on B. melitensis were investigated. In that study, the concentrations of 50, 100, 200 and 400 mg/mL of seed and leaf extracts caused growth inhibition zones of 5, 21, 24 and 26 mm and 6, 6, 6 and 7 mm in diameter, and its flower extract at all concentrations caused a growth inhibition zone of 6 mm in diameter. Finally, the root extract of the plant at 50, 100, 200 and 400 mg/mL was able to cause growth inhibition zones of 14, 19, 22 and 23 mm in diameter, respectively[82].

3.18. Prunus mahaleb & parsley (Petroselinum crispum)

Prunus mahaleb, a small tree with a height of 2-8 m, occurs in the central and southern Europe, and parsley is a turnip-rooted plant and grows more frequently in the cold and northern regions, especially in Europe and west Asia. The study of Seyyednejad et al. showed that the ethanol extract of Prunus mahaleb at 1.0 mg/mL could inhibit B. melitensis after 24 h incubation at 37 °C, with a minimum inhibitory concentration of 11 mm, but at higher concentrations, namely, 0.2 mg/mL, did not produce any effect. Ethanol parsley (Petroselinum crispum) seed extract at 0.1-0.2 mg/mL concentrations was also effective on B. melitensis, with a growth inhibition zone diameter of 7 and 9 mm after 24 h incubation, whereas at higher concentrations, did not produce any effect[83].

3.19. Quercus brantii (Q. brantii)

Q. brantii, spreading in west Asia and the Middle East, is a tall tree with a height of 20 m with oval leaves[84]. The study of Safary et al. showed that the methanol and ethanol extracts of Q. brantii fruit had a tremendous effects on B. melitensis, with an MIC of 30 and 26 mm for ethanol and methanol extracts at 0.44 g/mL, while the MIC of the studied antibiotic was 25 mm. Both ethanol and methanol extracts at 0.1, 0.2, 0.3, 0.4 and 0.5 g/mL were effective on bacteria, with MICs of 20, 25, 27, 30 and 15 mm and 20, 22, 25, 26 and 16 mm for 24 h at 37 °C[85].

3.20. Radix paeoniae Rubra, Coptis chinensis, Galla chinensis (G. chinensis) and Cortex phellodendrim

In a study, the effects of ethanol extract of various plants at 30 mg/mL on different species of B. melitensis including 16M, 293, 183, and 4611 were investigated. Overall, these plants showed anti-Brucella effects. Coptis chinensis, as a traditional drug called Huang-lian, has been widely used in Chinese and other Asian countries for the treatment and prevention of cancer and inflammation for centuries[86],[87],[88]. The whole dried root of Radix paeoniae is used as a traditional drug in China to treat blood stasis syndrome and to reduce pharygeal pain and pharyngitis[89]. and its MICs for the 16M, 293, 183, and 4611 species were 18, 14, 18 and 16 mm, respectively[90]. Different parts of the G. chinensis are used to treat various diseases in traditional Chinese medicine[91]. The MICs of the G. chinensis on species 16M, 293, 183 and 4611 were 39, 32, 33 and 40 mm, respectively[90]. Cortex phellodendrim, known as Huangbai, is used as a herbal drug in several regions of China and to treat diseases such as diarrhea and cancer[87]. The MICs of the Cortex phellodendrim on species 16M, 293, 183 and 4611 were 13, 12, 12 and 15 mm, respectively. Among the plants mentioned, G. chinensis produced the greatest effect against Brucella, so that the MIC of the antibiotic clavumox for the B. melitensis species M16 was 44 mm[90].

3.21. Saffron (Crocus sativus L.)

Crocus sativus belongs to the Iridaceae family and is found in the southern and central Europe, northern Africa, southwest Asia and west China[92]. The MICs of the ethanol and methanol extracts of the plant at 50, 100, 200 and 400 mg/mL were 8, 10, 12 and 14 mm and 8.9, 10.0, 12.0 and 14.0 mm, respectively[71].

3.22. Salvia sclarea

Salvia sclarea is a plant native to southern Europe with a height of 100 cm that is cultivated in France, Russia and the United States. The MICs of the ethanol and methanol extracts of the plant at 50, 100, 200 and 400 mg/mL were 7, 8, 10 mm and 12 and 6, 6, 9 and 14 mm, respectively[71].

3.23. Scrophularia deserti

Scrophularia deserti is found in Iran, Egypt, Palestine, Jordan and Saudi Arabia, grows up to 10-50 cm and has thick leaves[93]. The ethanol extract of the aerial parts of this plant showed various effects on Brucella at different concentrations. Ethanol extract of the plant at concentrations of 12, 24, 36, 48 and 60 μg/mL, caused growth inhibition zones of 6, 7, 7, 8 and 9 mm in diameter after 24 h, 6.5, 7.5, 7.5, 8.5 and 9.5 mm in diameter after 48 h, and 6.6, 7.7, 8.9, 9.0, and 9.7 mm in diameter after 72 h, respectively[94].

3.24. Teucrium polium

Teucrium polium is found in abundance and is used as a traditional drug in Iran. The plant has pink to white flowers[95]. The MICs of the ethanol and methanol extracts of this plant at 50, 100, 200 and 400 mg/mL were 6.0, 9.5, 10.0 and 12.0 mm, and 6, 8, 9 and 11 mm, respectively[71].

3.25. Tortille leptophylla (T. leptophylla)

T. leptophylla grows in Europe, North Africa, Southeast Asia and Iran with a height ranging from 20 cm to 70 cm. Maleki et al. reported that among the studied concentrations of ethanol T. leptophylla fruit extract, only 0.2 g/mL of the extract could affect B. melitensis within 24 h under 37 °C with a growth inhibition zone diameter of 8 mm, while the bacteria showed resistance to the dilutions of 0.3 and 0.1 g/mL[96].

3.26. Vitex pseudo-negundo

Vitex pseudo-negundo is found in different regions of the world from the Mediterranean region to central Asia and is used in Iran as a herbal drug[97]. The MIC of the ethanol extract of this plant at 50, 100, 200 and 400 mg/mL was 6 mm and those of the methanol extract 8, 8, 7 and 11, respectively[71].

3.27. Zataria multiflora Boiss (Z. multiflora), sumac and Ocimum basilicum L.

The effect of ethanol extracts of Z. multiflora, sumac and Ocimum basilicum on B. melitensis (Rev1) at 37 °C within 48 h was investigated. The first three plants showed anti-Brucella effects. Among the plants mentioned, Z. multiflora exhibited the strongest effect. Lamiaceae is a highly valuable medicinal plant and is found in large quantities in Iran, Pakistan and Afghanistan[98]. This plant is locally called Saatar, has small, egg-shaped and almost rounded leaves with a large number of small flowers[89],[99]. The plant at dilutions of 10, 20 and 40 mg/mL caused MICs of 11.11,18.88 and 28.77 mm, respectively[100]. Sumac is widely used in Turkey and the Middle East. The plant has red fruits with a single kernel, and the height of its tree reaches 1-4 m[101]. The plant also has had MICs of 6.77, 15.72, and 22.55 mm at dilutions of 10, 20 and 40 mg/mL, respectively[100]. The plant at concentrations of 20 and 40 mg/mL caused MICs of 6.66 and 7.5 mm[100].

3.28. Ziziphus spina-christi

Ziziphus spina-christi is a mountain shrub that grows widely and reaches a length of 5-10 m in the Mediterranean region, Africa, Australia and the tropical regions of the Americas[102]. Motamedi et al. showed that the ethanol and methanol extracts of Ziziphus spina-christi leaf had a strong effect on B. melitensis. The MICs of the ethanol and methanol extracts at concentrations of 0.1, 0.2, 0.3, 0.4 and 0.5 g/mL were 9, 10, 11, 15, 9 mm and 11, 11, 13, 15, 9 mm, respectively within 24 h[103].


  4. Conclusion Top


Perhaps the oldest human medicines for the treatment of undulant fever that still retain their place in modern medicine are herbal medicines. Three main properties of herbal drugs, including anti-inflammatory, antimicrobial and antioxidant, are often observed in the presented plants extracts and may be due to the presence of several compounds or a single compound. This is closely associated with some important issues in the treatment of brucellosis, such as the emergence of resistant microbial species to conventional antibacterial drugs. Medicinal plants have a wide range of active ingredients that can be used for specific treatment[104],[105],[106],[107],[108].

Although clinical evidence, in its current meaning, does not exist on all medicinal plants that are used traditionally, plants like Q. brantii fruit, O. acutidens seed, A. sativum, Peganum harmala, A. camelorum and some other plants have experimentally well established and their action mechanisms have been well explained pharmacologically[110], [110]. Physicians should not overlook the use of herbal drugs, but they should know the indications and ways of their administration, as well as the risks and limitations of their use. Herbal drugs in acute and severe diseases may often not comprise an adequate treatment, but they are nevertheless useful as complementary drugs. For example, the antimicrobial effects of medicinal plants as complementary drugs will be effective to control the acute infections that need to be treated with more potent drugs.

There is often no scientific evidence for the clinical effectiveness of herbal drugs, and more research is needed to confirm the effectiveness of medicinal plants. There is strong evidence on the antimicrobial effects of herbal drugs to protect the body against infection, but this is only very partially related to medical treatment and mostly includes dietary advice. However, a variety of plant extracts (ethanol, methanol, acetone, aqueous, etc.) and essential oils can be used to treat, control or increase the efficacy of the treatment.

Research on medicinal plants is always accompanied by identification of active ingredients. Plants reported in this article produce different antimicrobial effects, which is probably due to the difference in the active ingredients in the plants or the concentration of the active ingredient. For example, carvacrol is the main compound of plants such as O. acutidens, L. pubescens and O. syriacum. Carvacrol is a monoterpenoid phenol with a tangy taste. Carvacrol is produced by the plant’s aerial organs, and is stored in the subcuticular storage cavity[111]. The antibacterial action mechanism of carvacrol involves eliminating the membrane integrity, proton and potassium outflow from the cell by increasing the permeability of the membrane and changing pH gradient in the cells[112]. This substance can also depolarize the membrane by using its hydroxyl groups[113]. According to studies on the toxicity of this substance, the Caco-2 cells did not get damage in the short-term exposure to carvacrol[114]. Also, carvacrol has no significant effect on lymphocytes and indices of blood growth[115]. In addition, thymol is the main compound of plants such as Z. multiflora, Oliveria decumbens, Sylvia hortensis, N. cataria and T. syriacus. Thymol is the main identified phenol in the essential oils of the plants belonging to the Lamiaceae family[116]. Thymol is the product of p-cymene hydroxylation and p-cymene is the product of γ-terpinene aromatization[117],[118]. The essential oils rich in thymol are used in the food industry as a flavoring and preservative agent[119]. Thymol has also been used as an insect-repellent and for medical purposes from ancient times[120],[121]. It has a phenolic hydroxyl in different sites on the phenol ring and, by virtue of its hydrophilicity, dissolves and destroys the organisms, and also can lead to the depolarization of the bacterial membrane. Thymol produces no destructive effect on Caco-2 cells in the short term[114]. The main compound of some plants such as A. lappa, Q. brantii, G. chinensis, sumac and A. camelorum is tannin. Tannins are water-soluble polyphenols which can be detected in higher herbaceous and woody plants and are divided into two groups: Hydrolysable and non-hydrolysable. They are found in many foodstuffs such as tea, cocoa seed and grapes[122],[123]. Because of the presence of hydroxyl groups, they are able to bind to proteins and other macromolecules[124]. Tannins destroy bacteria through various mechanisms, including inhibition of bacterial enzymes through the formation of a complex with them, exerting destructive effect on the membrane of the organism, forming complex with iron ion, depriving the organism of its essential nutrients, and affecting the metabolism of the bacteria directly by inhibiting oxidative phosphorylation[125]. Other plants that have effective ingredients, such as berberine, possess many pharmaceutical and biological properties, such as anti-inflammatory, anti-tumor, viral, and bacterial, and stop bacterial growth by inhibiting DNA and protein synthesis, while causing very low toxicity[126],[127]. Therefore, the active ingredients of the herbal drugs can be used to make the best use of them. However, it is noteworthy that herbal remedies have different compounds that may lead to synergistic effects by inducing different mechanisms in the living organism and may be more effective than the constituents isolated from them.


  5. Recommendations Top


More clinical, controlled trials using extracts and other products are needed to determine the efficacy and risks of plant products in the treatment of brucellosis, as well as to examine the long-term safety and tolerability of plant medicines used in food supplements.

Conflict of interest statement

We declare that we have no conflict of interest.



 
  References Top

1.
Bayasgalan C, Chultemdorj T, Roth F, Zinsstag J, Hattendorf J, Badmaa B, et al. Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia. BMC Vet Res 2018; 14(1): 342.  Back to cited text no. 1
    
2.
Lai SH, Zhou H, Xiong W, Gilbert M, Huang ZH, Yu J, et al. Changing epidemiology of human brucellosis, China, 1955–2014. Emerg Infect Dis 2017; 23(2): 184-194.  Back to cited text no. 2
    
3.
Köse Ş, Serin Senger S, Akkoçlu G, Kuzucu L, Ulu Y, Ersan G, et al. Clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. Turk J Med Sci 2014; 44(2): 220-223.  Back to cited text no. 3
    
4.
Roushan MRH, Ebrahimpour S. Human brucellosis: An overview. Caspian J Intern Med 2015; 6(1): 46.  Back to cited text no. 4
    
5.
Mailles A, Garin-Bastuji B, Lavigne JP, Jay M, Sotto A, Maurin M, et al. Human brucellosis in France in the 21st century: Results from national surveillance 2004–2013. Med Mal Infect 2016; 46(8): 411-418.  Back to cited text no. 5
    
6.
Buzgan T, Karahocagil MK, Irmak H, Baran AI, Karsen H, Evirgen O, et al. Clinical manifestations and complications in 1028 cases of brucellosis: A retrospective evaluation and review of the literature. Int J Infec Dis 2010; 14(6): 469-478.  Back to cited text no. 6
    
7.
Rubach MP, Halliday JE, Cleaveland S, Crump JA. Brucellosis in low-income and middle-income countries. Curr Opin Infect Dis 2013; 26(5): 404.  Back to cited text no. 7
    
8.
Rossetti CA, Arenas-Gamboa AM, Maurizio E. Caprine brucellosis: A historically neglected disease with significant impact on public health. PLoS Negl Trop Dis 2017; 11(8): e0005692.  Back to cited text no. 8
    
9.
Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. Human gut microbiome viewed across age and geography. Nature 2012; 486(7402): 222.  Back to cited text no. 9
    
10.
Galinska EM, Zagórski J. Brucellosis in humans-etiology, diagnostics, clinical forms. Ann Agric Environ Med 2013; 20(2): 233-238.  Back to cited text no. 10
    
11.
Whatmore AM, Koylass MS, Muchowski J, Edwards-Smallbone J, Gopaul KK, Perrett LL. Extended multilocus sequence analysis to describe the global population structure of the genus Brucella: phylogeography and relationship to biovars. Front Microbiol 2016; 7: 2049.  Back to cited text no. 11
    
12.
Smith ME, Bhimji SS. Stat pearls. Treasure Island: Stat Pearls Publishing; 2018.  Back to cited text no. 12
    
13.
Seleem MN, Boyle SM, Sriranganathan N. Brucella: A pathogen without classic virulence genes. Vet Microbiol 2008; 129(1-2): 1-14.  Back to cited text no. 13
    
14.
Fontana C, Conde-Álvarez R, Ståhle J, Holst O, Iriarte M, Zhao Y, et al. Structural studies of lipopolysaccharide defective mutants from Brucella melitensis identify a core oligosaccharide critical in virulence. J Biol Chem 2016; 291(14):7727-7741.  Back to cited text no. 14
    
15.
Smith JA. Brucella Lipopolysaccharide and pathogenicity: The core of the matter. Virulence 2018; 9(1): 379-382.  Back to cited text no. 15
    
16.
Conde-Álvarez R, Arce-Gorvel V, Iriarte M, Manček-Keber M, Barquero-Calvo E, Palacios-Chaves L, et al. The lipopolysaccharide core of Brucella abortus acts as a shield against innate immunity recognition. PLoS Pathog 2012; 8(5): 1002675.  Back to cited text no. 16
    
17.
de Figueiredo P, Ficht TA, Rice-Ficht A, Rossetti CA, Adams LG. Pathogenesis and immunobiology of brucellosis: Review of Brucella–host interactions. Amer J Pathol 2015; 185(6): 1505-1517.  Back to cited text no. 17
    
18.
Skendros P, Pappas G, Boura P. Cell-mediated immunity in human brucellosis. Microbes Infect 2011; 13(2): 134-142.  Back to cited text no. 18
    
19.
Sangari FJ, Seoane A, Rodríguez MC, Agüero J, García Lobo JM. Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium. Infect Immun 2007; 75(2): 774-780.  Back to cited text no. 19
    
20.
Skendros P, Boura P. Immunity to brucellosis. Rev Sci Tech 2013; 32(1): 137-47.  Back to cited text no. 20
    
21.
Cui G, Wei P, Zhao Y, Guan Z, Yang L, Sun W, et al. Brucella infection inhibits macrophages apoptosis via Nedd4-dependent degradation of calpain2. Vet Microbiol 2014; 174(1-2): 195-205.  Back to cited text no. 21
    
22.
Wei P, Cui G, Lu Q, Yang L, Guan Z, Sun W, et al. A20 promotes Brucella intracellular growth via inhibition of macrophage cell death and activation. Vet Microbiol 2015; 175(1): 50-57.  Back to cited text no. 22
    
23.
Vitry MA, Mambres DH, Trez CD, Akira S, Ryffel B, Letesson JJ, et al. Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis. J Immunol 2014; 192(8): 3740-3752.  Back to cited text no. 23
    
24.
Aghaali M, Mohebi S, Heydari H. Prevalence of asymptomatic brucellosis in children 7 to 12 years old. Interdiscip Perspect Infect Dis 2015; 2015:187369.  Back to cited text no. 24
    
25.
Cascio A, De Caridi G, Lentini S, Benedetto F, Stilo F, Passari G, et al. Involvement of the aorta in brucellosis: The forgotten, life-threatening complication. A systematic review. Clin Infect Dis 2017; 64(8): 3-4.  Back to cited text no. 25
    
26.
Guven T, Ugurlu K, Ergonul O, Celikbas AK, Gok SE, Comoglu S, et al. Neurobrucellosis: clinical and diagnostic features. Clin Infec Dis 2013; 56(10): 1407-1412.  Back to cited text no. 26
    
27.
McGregor AC, Moore DA. Infectious causes of fever of unknown origin. Clin Med 2015; 15(3): 285-287.  Back to cited text no. 27
    
28.
Cunha BA, Lortholary O, Cunha CB. Fever of unknown origin: A clinical approach. Amer J Med 2015; 128(10): 1138.  Back to cited text no. 28
    
29.
Gul HC, Erdem H, Bek S. Overview of neurobrucellosis: A pooled analysis of 187 cases. Int J Infec Dis 2009; 13(6): 339-343.  Back to cited text no. 29
    
30.
Turan H, Serefhanoglu K, Karadeli E, Togan T, Arslan H. Osteoarticular involvement among 202 brucellosis cases identified in Central Anatolia region of Turkey. Intern Med 2011; 50(5): 421-428.  Back to cited text no. 30
    
31.
Gatselis NK, Makaritsis KP, Gabranis I, Stefos A, Karanikas K, Dalekos GN. Unusual cardiovascular complications of brucellosis presenting in two men: two case reports and a review of the literature. J Med Case Rep 2011; 5(1): 22.  Back to cited text no. 31
    
32.
Abid L, Frikha Z, Kallel S, Chokri Z, Ismahen B, Amin B, et al. Brucella myocarditis: A rare and life-threatening cardiac complication of Brucellosis. Intern Med 2012; 51(8): 901-904.  Back to cited text no. 32
    
33.
Jiang CQ, Shen LF, Feng Q, Wei F, Jiang RS, Zhang WW, et al. MRI features and categories of neurobrucellosis: A pooled review. Radiol Infect Dis 2018; 5(1): 1-6.  Back to cited text no. 33
    
34.
Erdem H, Senbayrak S, Meriç K, Batirel A, Karahocagil MK, Hasbun R, et al. Cranial imaging findings in neurobrucellosis: results of Istanbul-3 study. Infection 2016; 44(5): 623-631.  Back to cited text no. 34
    
35.
Chen GL, Feng TT, Xu ST, Wang JQ, Tan L, Wang JY, et al. A case report of brucellosis spondylitis with epidural abscess. Zhongguo Gu Shang 2017; 30(12):1151-1154.  Back to cited text no. 35
    
36.
Yemisen M, Karakas E, Ozdemir I, Karakas O. Brucellar testicular abscess: a rare cause of testicular mass. J Infect Chemother 2012; 18(5): 760-763.  Back to cited text no. 36
    
37.
Demiroğlu YZ, Turunç T, Alişkan H, Colakoğlu S, Arslan H. Brucellosis: Retrospective evaluation of the clinical, laboratory and epidemiological features of 151 cases. Mikrobiyol Bul 2007; 41(4): 517-527.  Back to cited text no. 37
    
38.
Demir C, Karahocagil MK, Esen R, Atmaca M, Gönüllü H, Akdeniz H. Bone marrow biopsy findings in brucellosis patients with hematologic abnormalities. Chin Med J (Engl) 2012; 125(11): 1871-1876.  Back to cited text no. 38
    
39.
Young EJ, Hasanjani Roushan MR, Shafae S, Genta RM, Taylor SL. Liver histology of acute brucellosis caused by Brucella melitensis. Human Pathol 2014; 45(10): 2023-2028.  Back to cited text no. 39
    
40.
Ozturk-Engin D, Erdem H, Gencer S, Kaya S, Baran AI, Batirel A, et al. Liver involvement in patients with brucellosis: results of the Marmara study. Eur J Clin Microbiol Infect Dis 2014; 33(7): 1253-1262.  Back to cited text no. 40
    
41.
Musallam II, Abo-Shehada MN, Hegazy YM, Holt HR, Guitian FJ. Systematic review of brucellosis in the Middle East: Disease frequency in ruminants and humans and risk factors for human infection. Epidemiol Infect 2016; 144(4): 671-685.  Back to cited text no. 41
    
42.
Ning C, Shuyi G, Tao Y, Hao Z, Zhang X. An epidemiological investigation of human brucellosis in Ulanqab, Inner Mongolia 2011. Chinese J Endemiol 2013; 32(6): 656-658.  Back to cited text no. 42
    
43.
Zhong Z, Yu S, Wang X, Dong S, Xu J, Wang Y, et al. Human brucellosis in the People’s Republic of China during 2005–2010. Int J Infect Dis 2013; 17(5): 289-292.  Back to cited text no. 43
    
44.
Wang Y, Zhang W, Ke Y, Zhen Q, Yuan X, Zou W. Human brucellosis, a heterogeneously distributed, delayed, and misdiagnosed disease in China. Clin Infect Dis 2013; 56(5): 750-751.  Back to cited text no. 44
    
45.
Ntirandekura JB, Matemba LE, Kimera SI, Muma JB, Karimuribo ED. Association of brucellosis with abortion prevalence in humans and animals in Africa: A review. Afr J Reprod Health 2018; 22(3): 120-136.  Back to cited text no. 45
    
46.
Adams DA. Summary of notifiable infectious diseases and conditions-United States, 2015. MMWR 2017; 64: 1-143.  Back to cited text no. 46
    
47.
Azadegan-Dehkordi F, Bagheri N, Shirzad H, Mahmoud Rafieian-Kopaei M. The role of Th1 and Th17 cells in glomerulonephritis. J Nephropathol 2015; 4(2): 32.  Back to cited text no. 47
    
48.
Zandi F. Evaluation of IL-17A and IL-17F genes polymorphism in Iranian dyspeptic patients. Life Sci J 2013; 10 (SPL. IS): 544-551.  Back to cited text no. 48
    
49.
Menbari MN, Rahmani SA, Ahmadi A, Sanandaj I. Evaluation of E-cadherin (CDH1) gene polymorphism related to gastric cancer in Kurdish population. Life Sci J 2013; 10(12): 212-216.  Back to cited text no. 49
    
50.
Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL. Trends in the use of complementary health approaches among adults: United States, 2002–2012. Natl Health Stat Report 2015; 10(79): 1-16.  Back to cited text no. 50
    
51.
Gurrapu S, Mamidala E. Medicinal plants used by traditional medicine practitioners in the management of HIV/AIDS-related diseases in tribal areas of Adilabad district, Telangana region. Am J Sci Med Res 2016; 2(1): 239-245.  Back to cited text no. 51
    
52.
Asadi-Samani M, Kooti W, Aslani E, Shirzad H. A systematic review of Iran’s medicinal plants with anticancer effects. Evid Based Compl Alt Med 2016; 21(2): 143-153.  Back to cited text no. 52
    
53.
Honari M, Askari H, Khosrowchahli M. Use of desirability function method in optimization of regeneration and callus induction of Alhagi camelorum. Am J Plant Sci 2014; 5(03): 268.  Back to cited text no. 53
    
54.
Ghasemi Pirbalouti, Ghasemi MR, Momtaz H, Golparvar AR, Hamedi B, Shahgholian L. The effect of some of the Iranian medicinal plants on Brucella abortus on in vitro and in vivo. J Herb Drugs 2010; 1(1): 21-29.  Back to cited text no. 54
    
55.
Wu C, Wang M, Dong Y, Cheng ZH, Meng H. Growth, bolting and yield of garlic (Allium sativum L.) in response to clove chilling treatment. Sci Horticul 2015; 194: 43-52.  Back to cited text no. 55
    
56.
Shapoury R, Sattari M, Mohammad Hassan Z, Study effect of garlic choloroformic extract (Allicin) on physiology and morphology of Brucella. J Med Plants 2004; 2(10): 15-22.  Back to cited text no. 56
    
57.
Branch Z. Evaluation of the antibacterial effects of Arctium lappa extracts on Brucella melitensis 16M in the animal model and macrophage culture. J Mil Med 2016; 18(1): 315-322.  Back to cited text no. 57
    
58.
Ashraf H, Heidari R, Nejati V. Antihyperglycemic and antihyperlipidemic effects of fruit aqueous extract of Berberis integerrima Bge. in streptozotocin-induced diabetic rats. Iran J Pharmac Res 2014; 13(4): 1313.  Back to cited text no. 58
    
59.
Azimi G, Hakakian A, Ghanadian M, Joumaa A, Alamian S. Bioassay-directed isolation of quaternary benzylisoquinolines from Berberis integerrima with bactericidal activity against Brucella abortus. Res Pharmac Sci 2018; 13(2): 149.  Back to cited text no. 59
    
60.
Morcia C, Tumino G, Ghizzoni R, Terzi V. Carvone (Mentha spicata L.) oils. In: Prredy VR (editor). Essential oils in food preservation, flavor and safety. Chapter 35. London: Academic Press; 2016, p.309-316.  Back to cited text no. 60
    
61.
Singh BR, Agrawal RK, Singh KP, Pawde AM, Sinha DK, Dubey S, et al. Antibacterial activity of Caraway essential oil against bacteria isolated from veterinary clinical cases. Nat Prod: An Indian J 2015; 11: 69-74.  Back to cited text no. 61
    
62.
Mahendiran M, Umavathi S. In vitro antimicrobial activity of Citrullus colocynthis (Linn.) against selected microorganisms. Int J Curr Microbiol App Sci 2015; 4(10): 60-69.  Back to cited text no. 62
    
63.
Ray J, Goyal P, Aggarwal BK. Approach of Eucalyptus globulus plant parts for human health safety and toxicological aspects. Brit Open J Plant Sci 2015; 1(1): 1-10.  Back to cited text no. 63
    
64.
Abdolahzade P, Shapouri R, Nasiri Semnani S. Antibacterial effects of eucalyptus globulus extracts on Brucella melitensis M16 and Brucella abortus s99 in vitro and in vivo. J Ardabil Uni Med Sci 2011; 11(3): 218-227.  Back to cited text no. 64
    
65.
Olšovská J, Vanda Boštíková V, Dušek M, Jandovská V, Bogdanová K, Èermák P, et al. Humulus lupulus L. Hops: A valuable source of compounds with bioactive effects for future therapies. Mil Med Sci Lett 2016; 85(1): 19-30.  Back to cited text no. 65
    
66.
Shapouri R, Rahnema M. Evaluation of antimicrobial effect of hops extracts on intramacrophages Brucella abortus and B. melitensis. Jundishapur J Microbiol 2011; 4(5): 51-58.  Back to cited text no. 66
    
67.
Karaman I, Sahin F, Güllüce M, Ögütçü H, Sengül M, Adıgüzel A. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 2003; 85(2-3): 231-235.  Back to cited text no. 67
    
68.
Al-Mariri A, Saour G, Hamou R. In vitro antibacterial effects of five volatile oil extracts against intramacrophage Brucella abortus 544. Iran J Med Sci 2012; 37(2): 119.  Back to cited text no. 68
    
69.
Rattanachaikunsopon P, Phumkhachorn P. Potential of cinnamon (Cinnamomum verum) oil to control Streptococcus iniae infection in tilapia (Oreochromis niloticus). Fish Sci 2010; 76(2): 287-293.  Back to cited text no. 69
    
70.
Neeraj T, Prakash A, Seema Y. Antimicrobial activity and medicinal values of essential oil of Mentha piperita L. Int J Eng Innov Technol 2013; 2(8): 214-8.  Back to cited text no. 70
    
71.
Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad SM. In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Uni Sci B 2010; 11(7): 506-511.  Back to cited text no. 71
    
72.
Rashed MM, Tong Q, Abdelhai MH, Gasmalla MA, Ndayishimiye JB, Chen L, et al. Effect of ultrasonic treatment on total phenolic extraction from Lavandula pubescens and its application in palm olein oil industry. Ultrason Sonochem 2016; 29: 39-47.  Back to cited text no. 72
    
73.
Adiguzel A, Ozer H, Sokmen M, Gulluce M, Sokmen A, Kilic H, et al. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. Polish J Microbiol 2009; 58(1): 69-76.  Back to cited text no. 73
    
74.
Butt MS, Sultan MT. Nigella sativa: reduces the risk of various maladies. Crit Rev Food Sci Nutr 2010; 50(7): 654-665.  Back to cited text no. 74
    
75.
Alnaemi H, Alsawaf S. Effect of Nigella sativa (seed and oil) on the bacteriological quality of soft white cheese. Iraqi J Vet Sci 2011; 25(1): 21-27.  Back to cited text no. 75
    
76.
Sereshti H, Izadmanesh Y, Samadi S. Optimized ultrasonic assisted extraction–dispersive liquid–liquid microextraction coupled with gas chromatography for determination of essential oil of Oliveria decumbens Vent. J Chromatogr A 2011; 1218(29): 4593-4598.  Back to cited text no. 76
    
77.
Sökmen M, Serkedjieva J, Daferera D, Gulluce M, Polissiou M, Tepe B, et al. In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J Agric Food Chem 2004; 52(11): 3309-3312.  Back to cited text no. 77
    
78.
Zayzafoon G, Odeh O, Allaf AW. Determination of essential oil composition by GC-MS and integral antioxidant capacity using photochemiluminescence assay of two Thymus leaves: Thymus syriacus and Thymus cilicicus from different Syrian locations. Herba Pol 2012; 58(4): 71-84.  Back to cited text no. 78
    
79.
Al-Mariri A, Swied GH, Oda A, Al Hallab L. Antibacterial activity of Thymus syriacus boiss essential oil and its components against some Syrian gram-negative bacteria isolates. Iran J Med Sci 2013; 38(2 Suppl): 180.  Back to cited text no. 79
    
80.
Al-Mariri A, Safi M. The antibacterial activity of selected labiatae (Lamiaceae) essential oils against Brucella melitensis. Iran J Med Sci 2013; 38(1): 44.  Back to cited text no. 80
    
81.
Yousefi R, Ghaffarifar F, Asl AD. The effect of Alkanna tincturia and Peganum harmala extracts on Leishmania major (MRHO/IR/75/ER) in vitro. Iran J Parasitol 2009; 4(1): 40-47.  Back to cited text no. 81
    
82.
Darabpour E, Poshtkouhian Bavi A, Motamedi H, Seyyed Nejad SM. Antibacterial activity of different parts of Peganum harmala L. growing in Iran against multi-drug resistant bacteria. EXCLI J 2011; 10: 252.  Back to cited text no. 82
    
83.
Seyyednejad S. Antibacterial activity of Prunus mahaleb and Parsley (Petroselinum crispum) against some pathogen. Asian J Biol Sci 2008; 1(1): 51-55.  Back to cited text no. 83
    
84.
Bahar ZR, Ghotaslou R, Taheri S. In vitro anti-biofilm activity of Quercus brantii subsp. persica on human pathogenic bacteria. Res J Pharmacog 2017; 4(1): 67-73.  Back to cited text no. 84
    
85.
Safary A, Motamedi H, Maleki S, Seyyednejad SM. A preliminary study on the antibacterial activity of Quercus brantii against bacterial pathogens, particularly enteric pathogens. Intern J Bot 2009; 5(2):176-180.  Back to cited text no. 85
    
86.
Zhang Q, Piao XL, Piao XS, Lu T, Wang D, Kim SW. Preventive effect of Coptis chinensis and berberine on intestinal injury in rats challenged with lipopolysaccharides. Food Chem Toxicol 2011; 49(1): 61-69.  Back to cited text no. 86
    
87.
Zhu SL, Dou SS, Liu XR, Liu RH, Zhang WD, Huang HL, et al. Qualitative and quantitative analysis of alkaloids in Cortex phellodendri by HPLC-ESI-MS/MS and HPLC-DAD. Chem Res Chin Univ 2011; 27(1): 38-44.  Back to cited text no. 87
    
88.
Wen KW, Bejo K. Screening of Chinese medicinal herbs for the inhibition of Brucella melitensis. J Ethnopharmacol 2008; 120(2): 287-290.  Back to cited text no. 88
    
89.
Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss.(Shirazi thyme): An ancient condiment with modern pharmaceutical uses. J Ethnopharmacol 2013; 145(3): 686-698.  Back to cited text no. 89
    
90.
Prieto JM, Recio MC, Giner RM, Máñez S, Giner-Larza EM, Ríos JL. Influence of traditional Chinese antiinflammatory medicinal plants on leukocyte and platelet functions. J Pharm Pharmacol 2003; 55(9): 1275-1282.  Back to cited text no. 90
    
91.
Djakpo O, Yao W. Rhus chinensis and Galla Chinensis–folklore to modern evidence. Phytother Res 2010; 24(12): 1739-1747.  Back to cited text no. 91
    
92.
Petersen G, Seberg O, Thorsøe S, Jørgensen T, Mathew B. A phylogeny of the genus Crocus (Iridaceae) based on sequence data from five plastid regions. Taxon 2008; 57(2): 487-499.  Back to cited text no. 92
    
93.
Bahmani M, Ghorbani M, Momtaz H, Rafieian M. The comparison of the in-vitro effects of Scrophularia deserti plant and amphotricin B on Candida albicans. Arak Univ Med Sci J 2011; 13(4): 15-21.  Back to cited text no. 93
    
94.
Bahmani M, Vakili Saatloo N, Maghsoudi R, Momtaz H, Saki K, Kazemi-Ghoshchi B, et al. A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis. J Herb Med Pharmacol 2013; 2(1): 17-20.  Back to cited text no. 94
    
95.
Haghighi M and Mozafariyan M. The introduction of extinct endemic vegetables of Iran. J Med Plants Res 2011; 5(33): 7085-7107.  Back to cited text no. 95
    
96.
Maleki S, Seyyednejad SM, Damabi NM, Motamedi H. Antibacterial activity of the fruits of Iranian Torilis leptophylla against some clinical pathogens. Pak J Biol Sci 2008; 11(9): 1286-1289.  Back to cited text no. 96
    
97.
Mozdianfard M, Akhbari M, Batooli H. Comparative study on the antioxidant activities of the different extracts and the composition of the oil extracted by n-hexane from Iranian Vitex pseudo-negundo. Nat Prod Res 2012; 26(23): 2162-2167.  Back to cited text no. 97
    
98.
Hadian J, Ebrahimi SN, Mirjalili MH, Azizi A, Ranjbar H, Friedt W. Chemical and genetic diversity of Zataria multiflora Boiss. accessions growing wild in Iran. Chem Biodivers 2011; 8(1): 176-188.  Back to cited text no. 98
    
99.
Khosravi AR, Shokri H, Sharifrohani M, Mousavi HE, Moosavi Z. Evaluation of the antifungal activity of Zataria multiflora, Geranium herbarium and Eucalyptus camaldolensis essential oils on Saprolegnia parasitica–infected rainbow trout (Oncorhynchus mykiss) eggs. Foodborne Pathog Dis 2012; 9(7): 674-679.  Back to cited text no. 99
    
100.
Motaharinia Y, Rezaee MA, Hazhir MS, Rahmani MR. Evaluation of the antibacterial activity of Zataria multiflora Boiss., Rhus coriaria L.(sumac), Mentha piperita L., and Ocimum basilicum L. extracts on Brucella strains isolated from brucellosis patients. Turk J Med Sci 2012; 42(5): 816-822.  Back to cited text no. 100
    
101.
Mahboubi M. Iranian medicinal plants as antimicrobial agents. J Microbiol, Biotechnol Food Sci 2013; 2(4): 2388.  Back to cited text no. 101
    
102.
Saied AS, Gebauer J, Hammer K, Buerkert A. Ziziphus spina-christi (L.) Willd: A multipurpose fruit tree. Genetic Resour Crop Evol 2008; 55(7): 929-937.  Back to cited text no. 102
    
103.
Motamedi H, Safary A, Maleki S, Seyyednejad SM. Ziziphus spina-christi, a native plant from Khuzestan, Iran, as a potential source for discovery new antimicrobial agents. Asian J Plant Sci 2009; 8(2): 187.  Back to cited text no. 103
    
104.
Fallaha S, Rostaeia M, Lorigooini Z, Abbasi Surki A. Chemical compositions of essential oil and antioxidant activity of dragonhead (Dracocephalum moldavica) in sole crop and dragonhead-soybean (Glycine max) intercropping system under organic manure and chemical fertilizers. Ind Crop Prod 2018; 115: 158-165.  Back to cited text no. 104
    
105.
Behruzian A, Hosseinzadeh Samani B, Rostami S, Lorigooini Z, Behruzian M. The effect of combined AC electric field and ultrasound on the chemical compositions and Escherichia coli content of spearmint aromatic water. J Food Proc Eng 2018; 41(2): e12650.  Back to cited text no. 105
    
106.
Ghasemi S, Lorigooini Z, Wibowo J, Amini-Khoei H. Tricin isolated from Allium atroviolaceum potentiated the effect of docetaxel on PC3 cell proliferation: Role of miR-21. Nat Prod Res 2018; 1-4.  Back to cited text no. 106
    
107.
Saeidi K, Mossavi M, Lorigooini Z, Maggi F. Chemical characterization of the essential oil compositions and antioxidant activity from Iranian populations of Achillea wilhelmsii K. Koch. Ind Crop Prod 2018; 112: 274-280.  Back to cited text no. 107
    
108.
Jamshidi-Kia F, Lorigooini Z, Asgari S, Saidi K. Iranian species of Verbascum: A review of botany, phytochemistry, and pharmacological effects. Toxin Rev 2018: doi.org/10.1080/15569543.2018.1457055.  Back to cited text no. 108
    
109.
Torki A, Khalaji-Pirbalouty V, Lorigooini Z, Rafieian-Kopaei M, Sadeghimanesh A, Rabiei Z. Anchusa italica extract: phytochemical and neuroprotective evaluation on global cerebral ischemia and reperfusion. Braz J Pharm Sci 2018; 54(1): http://dx.doi.org/10.1590/s2175-97902018000117251.  Back to cited text no. 109
    
110.
Hosseinzadeh Samani B, Gudarzi H, Rostami S, Lorigooini Z, Esmaeili Z, Jamshidikia F. Development and optimization of the new ultrasonic-infrared-vacuum dryer in drying Kelussia odoratissima and its comparison with conventional methods. Indus Crop Prod 2018; 123(1): 46-54.  Back to cited text no. 110
    
111.
Suganthi RU, Manpal S. Biological and pharmacological of actions carvacrol and its effects on poultry: An updated review. World J Pharm Pharm Sci 2013; 2: 3581-95.  Back to cited text no. 111
    
112.
Xu J, Zhou F, Ji BP, Pei RS, Xu N. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Lett Appl Microbiol 2008; 47(3): 174-179.  Back to cited text no. 112
    
113.
Burt SA, Ojo-Fakunle VT, Woertman J, Veldhuizen EJ. The natural antimicrobial carvacrol inhibits quorum sensing in Chromobacterium violaceum and reduces bacterial biofilm formation at sub-lethal concentrations. PLoS One 2014; 9(4): e93414.  Back to cited text no. 113
    
114.
Fabian D, Sabol M, Domaracká K, Bujnáková D. Essential oils: Their antimicrobial activity against Escherichia coli and effect on intestinal cell viability. Toxicol Nitro 2006; 20(8): 1435-1445.  Back to cited text no. 114
    
115.
Nofrarías M, Manzanilla EG, Pujols J, Gibert X, Majó N, Segalés J, et al. Effects of spray-dried porcine plasma and plant extracts on intestinal morphology and on leukocyte cell subsets of weaned pigs. J Anim Sci 2006; 84(10): 2735-2742.  Back to cited text no. 115
    
116.
Tuttolomondo T, Dugo G, Ruberto G, Leto C, Napoli EM, Cicero N, et al. Study of quantitative and qualitative variations in essential oils of Sicilian Rosmarinus officinalis L. Nat Prod Res 2015; 29(20): 1928-1934.  Back to cited text no. 116
    
117.
Rowshan V, Bahmanzadegan A, Saharkhiz MJ. Influence of storage conditions on the essential oil composition of Thymus daenensis Celak. Ind Crop Prod 2013; 49: 97-101.  Back to cited text no. 117
    
118.
Nikolić M, Glamoclijia J, Ferreira Isabel CFR, Calhelda RC, Fernandes A, Markovic T, et al. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. Reut and Thymus vulgaris L. essential oils. Ind Crop Prod 2014; 52: 183-190.  Back to cited text no. 118
    
119.
Marchese A, Orhan IE, Daglia M, Barbieri R, Di Lorenzo A, Nabavi SF, et al. Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chem 2016; 210: 402-414.  Back to cited text no. 119
    
120.
Giatropoulos A, Kimbaris A, Michaelakis A, Papachristos DP, Polissiou MG, Emmanouel N. Chemical composition and assessment of larvicidal and repellent capacity of 14 Lamiaceae essential oils against Aedes albopictus. Parasitol Res 2018; 117(6): 1953-1964.  Back to cited text no. 120
    
121.
Nesterkina M, Bernier UR, Tabanca N, Kravchenko I. Repellent activity of monoterpenoid esters with neurotransmitter amino acids against yellow fever mosquito, Aedes aegypti. Open Chem 2018; 16(1): 95-98.  Back to cited text no. 121
    
122.
Khasnabis J, Rai C, Roy A. Determination of tannin content by titrimetric method from different types of tea. J Chem Pharm Res 2015; 7(6): 238-241.  Back to cited text no. 122
    
123.
Barrett A, Ndou T, Hughey CA, Straut C, Howell A, Dai Z, et al. Inhibition of α-amylase and glucoamylase by tannins extracted from cocoa, pomegranates, cranberries, and grapes. J Agric Food Chem 2013; 61(7): 1477-1486.  Back to cited text no. 123
    
124.
Melone F, Saladino R, Lange H, Crestini C. Tannin structural elucidation and quantitative 31P NMR analysis. 2. Hydrolyzable tannins and proanthocyanidins. J Agric Food Chem 2013; 61(39): 9316-9324.  Back to cited text no. 124
    
125.
Macáková K, Kole V, Cahlíková L, Chlebek J, Hošt'álková A, Kuca K, et al. Tannins and their influence on health. Recent Adv Med Chem 2015; 1: 159-208.  Back to cited text no. 125
    
126.
Kang S, Li Z, Yin Z, Jia R, Song X, Li L, et al. The antibacterial mechanism of berberine against Actinobacillus pleuropneumoniae. Nat Prod Res 2015; 29(23):2203-6.  Back to cited text no. 126
    
127.
Kong WJ, Xing XY, Xiao XH, Zhao YL, Wei JH, Wang JB, Yet al. Effect of berberine on Escherichia coli, Bacillus subtilis, and their mixtures as determined by isothermal microcalorimetry. Appl Microbiol Biotechnol 2012; 96(2): 503-510.  Back to cited text no. 127
    


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1]



 

Top
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  3. Plants used i...
  In this article
Abstract
1. Introduction
2. Retrieve methods
4. Conclusion
5. Recommendations
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed229    
    Printed12    
    Emailed0    
    PDF Downloaded109    
    Comments [Add]    

Recommend this journal