Impact Factor 2017: 1.634 (@Clarivate Analytics)
5-Year Impact Factor: 1.677 (@Clarivate Analytics)
  • Users Online: 13
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2018  |  Volume : 11  |  Issue : 10  |  Page : 590-596

Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847)


1 Department of Biology, Faculty of Science; Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand
2 Department of Biology, Faculty of Science; Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science; Environmental Science Research Center, Faculty of Science; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand

Correspondence Address:
Nattawadee Nantarat
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202
Thailand
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1995-7645.244524

Get Permissions

Objective: To investigate the parasitic infection of Anentome helena (A. helena) and determine the validity of species boundaries for A. helena by combining molecular phylogeny and morphological approaches. Methods: A total of 325 individuals of A. helena were collected throughout northern Thailand. Shells were measured and compared by t-test. Radulae were investigated by using light and scanning electron microscope. Two partial mitochondrial DNA sequences of COI and 16S rRNA from 36 specimens of A. helena and related species were used to test the validity of the morphospecies. Phylogenetic trees were constructed using neighbour joining, maximum likelihood and Bayesian inference. Infection of A. helena with trematode larva was examined and observed. Results: Morphological examination of A. helena revealed 2 distinct morphospecies. Genetic divergences supported the separation of the two morphotypes into two distinct groups. Both individual and combined analyses of the two nucleotide fragments revealed two phylogroups that corresponded with shell and radula characteristics. In addition, A. helena was found infected with 37-collar spined echinostome metacercariae. The prevalence and intensity of metacercariae was highest in the San Kamphaeng district, Chiang Mai province, with 7.5% and 1.670±0.577, respectively. Conclusions: These findings suggest that comprehensive taxonomic revision of this unrecognised species complex is needed. This study represents the preliminary step to reveal new data on the recent distribution of trematode infection in A. helena. This information may be useful for developing conservation management of the snail and the practice of targeted regimes to reduce anthelmintic resistance in the future.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed116    
    Printed4    
    Emailed0    
    PDF Downloaded29    
    Comments [Add]    

Recommend this journal